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Human Fc gamma RIII (CD16) PerCP-conjugated Antibody

Catalog #: FAB2546C Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2546C-025
FAB2546C-100
Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry.
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Human Fc gamma RIII (CD16) PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed.
Source
Monoclonal Mouse IgG2A Clone # 245536
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16)
Thr20-Gln208
Accession # O75015
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Fc gamma RIII (CD16) antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Fc gamma RIII (CD16) PerCP-conjugated Monoclonal Antibody (Catalog # FAB2546C) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC003C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RIII (CD16)

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

Long Name
Fc gamma Receptor III
Entrez Gene IDs
14131 (Mouse); 102140945 (Cynomolgus Monkey)
Alternate Names
CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20

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