Human Fc gamma RII/CD32 APC-conjugated Antibody

Catalog #: FAB1330A Datasheet / COA / SDS

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FAB1330A

Detection of Fc gamma RII/CD32 in Human Lymphocytes by Flow Cytometry.

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Human Fc gamma RII/CD32 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fc gamma RIIA and human Fc gamma RIIB in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse Fc gamma RII.

Source

Monoclonal Mouse IgG_2A Clone # 190723

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB
Ala46-Pro217
Accession # P31994

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Fc gamma RII/CD32 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Fc gamma RII/CD32 APC-conjugated Monoclonal Antibody (Catalog # FAB1330A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Fc gamma RII/CD32

Fc gamma RII, also known as CD32, is a group of three closely related proteins (Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIC) that share greater than 94% amino acid identity in their extracellular domains. They function as transmembrane receptors for the Fc portion of IgG molecules. These proteins are expressed by various hematopoietic cells including monocytes, macrophages, neutrophils, NK, T cells, and B cells. The Fc gamma RII proteins are involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name

Fc gamma Receptor II

Entrez Gene IDs

2212 (Human); 14130 (Mouse)

Alternate Names

CD32 antigen; CD32B; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RII; Fc gamma RIIb; FCG2; Fc-gamma RII-b; FcgammaRIIb; fc-gamma-RIIb; FCGR2; FcgRII; FcgRIIB; fcRII-b; FcRII-c; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b