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Human Fc gamma RIIIA/B (CD16) Antibody

Catalog #: AF1597
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1597
AF1597-SP
Detection of Human Fc gamma RIII (CD16) by Western Blot.
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Fc gamma RIIIA/B (CD16) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant human Fc gamma  RIIA is observed and less than 2% cross-reactivity with recombinant mouse CD16 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIB (R&D Systems, Catalog # 1597-FC)
Thr20-Gln208
Accession # O75015
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 5 µg/mL of human IgG to immobilized Recombinant Human Fc gamma  RIIIB/CD16b (Catalog # 1597-FC) coated at 5 µg/mL (100 µL/well). At 50 µg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Fc gamma RIII (CD16) antibody by Western Blot. View Larger

Detection of Human Fc gamma RIII (CD16) by Western Blot. Western blot shows lysates of human spleen tissue, human lung tissue, and human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Fc gamma RIII (CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1597) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fc gamma RIII (CD16) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIII (CD16)

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta  chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells, and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Signaling through Fc gamma RIIIA results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells. By contrast, Fc gamma RIIIB is a decoy receptor that binds IgG complexes without triggering activation. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes (4). It interacts with complement receptors CR3 and CR4 on monocytes to induce the production of pro-inflammatory cytokines.

References
  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Gauchat, G.J. et al. (1996) J. Immunol. 157:1184.
Long Name
Fc gamma Receptor III
Entrez Gene IDs
14131 (Mouse); 102140945 (Cynomolgus Monkey)
Alternate Names
CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20

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Citations for Human Fc gamma RIIIA/B (CD16) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. One N-glycan regulates natural killer cell antibody-dependent cell-mediated cytotoxicity and modulates Fc ? receptor IIIa/CD16a structure
    Authors: Kremer, PG;Lampros, EA;Blocker, AM;Barb, AW;
    eLife
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Immune checkpoint markers and anti-CD20-mediated NK cell activation
    Authors: Zhaoming Wang, George J. Weiner
    Journal of Leukocyte Biology
  3. Fc &gamma receptor IIIa / CD16a processing correlates with the expression of glycan-related genes in human natural killer cells
    Authors: KR Patel, MC Rodriguez, WW Lorenz, EM Mace, AW Barb
    The Journal of Biological Chemistry, 2020-12-16;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Inhibition of osteoclastogenesis by opsonized Porphyromonas gingivalis
    Authors: Subramanya N. Pandruvada, Jeffrey L. Ebersole, Sarandeep S. Huja
    FASEB BioAdvances
  5. Multivalent Fc?-receptor engagement by a hexameric Fc-fusion protein triggers Fc?-receptor internalisation and modulation of Fc?-receptor functions
    Authors: OS Qureshi, TF Rowley, F Junker, SJ Peters, S Crilly, J Compson, A Eddleston, H Björkelund, K Greenslade, M Parkinson, NL Davies, R Griffin, TL Pither, K Cain, L Christodou, L Staelens, E Ward, J Tibbitts, A Kiessling, B Smith, FR Brennan, M Malmqvist, F Fallah-Ara, DP Humphreys
    Sci Rep, 2017-12-06;7(1):17049.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  6. Microvesicles released by apoptotic human neutrophils suppress proliferation and IL-2/ IL-2 receptor expression of resting T helper cells
    Authors: G Shen, S Krienke, P Schiller, A Nie beta en, S Neu, V Eckstein, M Schiller, HM Lorenz, LO Tykocinski
    Eur. J. Immunol, 2017-04-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Induced expression of FcgammaRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-gammahigh subset.
    Authors: Chauhan A, Chen C, Moore T, DiPaolo R
    J Biol Chem, 2015-01-02;290(8):5127-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Immune complexes and late complement proteins trigger activation of Syk tyrosine kinase in human CD4(+) T cells.
    Authors: Chauhan AK, Moore TL
    Clin. Exp. Immunol., 2012-02-01;167(2):235-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Alpha-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8.
    Authors: Bergin DA, Reeves EP, Meleady P, Henry M, McElvaney OJ, Carroll TP, Condron C, Chotirmall SH, Clynes M, O'Neill SJ, McElvaney NG
    J. Clin. Invest., 2010-11-08;120(12):4236-50.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot

FAQs

  1. In the blockade of receptor-ligand interation assay, does Human Fc gamma RIIIA/B (CD16) Antibody, Catalog # AF1597 bind Recombinant Human Fc gamma RIIIB/CD16b Protein, Catalog # 1597-FC,which is a Fc receptor, via its FAB (antigen binding) domain or Fc (constant region) domain?

    • We have tried AF1597 in our protocol side by side with Normal Goat IgG (Catalog # AB-108-C) as a negative control. Normal goat IgG did not block the receptor-ligand interaction. This indirectly indicates that AF1597 binds 1597Fc via is FAB region.

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