Human Fc gamma RIIIB/CD16b Antibody

Name: Human Fc gamma RIIIB/CD16b Antibody
Brand: R&D Systems
SKU: MAB15971
Price: 419 USD

Catalog #: MAB15971 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Discontinued Product

MAB15971 has been discontinued.

View all Fc gamma RIIIB/CD16b products.

Detection of Fc gamma RIIIB/CD16b in Human Peripheral Blood Granulocytes by Flow Cytometry.

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All Fc gamma RIIIB/CD16b Antibodies

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Human Fc gamma RIIIB/CD16b Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fc gamma RIIIB/CD16b in Elisas.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2830D

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293-derived human Fc gamma RIIIB/CD16b protein
Met18-Ser200
Accession # NP_000561

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human peripheral blood cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Fc gamma RIIIB/CD16b in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) Rabbit Anti-Human Fc gamma RIIIB/CD16b Monoclonal Antibody (Catalog # MAB15971) or (B) Rabbit IgG isotype control antibody (MAB1050) followed by Goat anti-Rabbit IgG APC-conjugated Secondary Antibody (F0111) and Mouse Anti-Human Siglec-8 PE-conjugated Monoclonal Antibody (FAB7975P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIIB/CD16b

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

Long Name

Fc gamma Receptor III

Entrez Gene IDs

2215 (Human); 14131 (Mouse)

Alternate Names

CD16; CD16b antigen; CD16b; Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc fragment of IgG, low affinity IIIb, receptor for (CD16); Fc gamma RIIIB; FCG3; Fc-gamma receptor IIIb (CD 16); Fc-gamma RIII; Fc-gamma RIIIb; Fc-gamma RIII-beta; FCGR3; FCGR3B; FcgRIIIB; FcR-10; fcRIII; FCRIIIB; IGFR3; IgG Fc receptor III-1; low affinity immunoglobulin gamma Fc region receptor III-B