Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human FGF acidic/FGF1 Quantikine ELISA Kit Summary
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
Sensitivity
13.9 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human FGF acidic
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human FGF acidic Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF acidic in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF acidic and has been shown to accurately quantitate the recombinant factor.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 208 | 485 | 766 | 204 | 477 | 761 |
| Standard Deviation | 7.6 | 21.8 | 22.9 | 15 | 29.3 | 48.7 |
| CV% | 3.7 | 4.5 | 3 | 7.4 | 6.1 | 6.4 |
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 179 | 441 | 749 | 178 | 433 | 713 |
| Standard Deviation | 8.3 | 31.8 | 17.6 | 15 | 36.3 | 61 |
| CV% | 4.6 | 7.2 | 2.3 | 8.4 | 8.4 | 8.6 |
Recovery
The recovery of FGF acidic spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 93-113 |
| EDTA Plasma (n=4) | 102 | 93-109 |
| Heparin Plasma (n=4) | 97 | 92-110 |
| Serum (n=4) | 98 | 89-108 |
| Urine (n=4) | 99 | 89-108 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of FGF acidic were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF acidic/FGF1
FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF acidic is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF acidic can translocate to the cytosol and to the nucleus. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.
Long Name:
Fibroblast Growth Factor acidic
Entrez Gene IDs:
2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
Alternate Names:
AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 150 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
150 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human FGF acidic/FGF1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
Filter your results:
Filter by:
-
The EGFR-STYK1-FGF1 axis sustains functional drug tolerance to EGFR inhibitors in EGFR-mutant non-small cell lung cancer
Authors: C Eggermont, P Giron, M Noeparast, H Vandenplas, P Aza-Blanc, GJ Gutierrez, J De Grève
Oncogene, 2022-07-15;13(7):611.
Species: Human
Sample Types: Cell Lysates
-
Validation of a Double-Sandwich Enzyme-Linked Immunoassay for Pharmacokinetic Study of an rh-aFGF Hydrogel in Rat Skin and Serum
Authors: Q Hui, R Yang, C Lu, J Bi, L Li, J Gong, L Zhang, Z Jin, X Li, X Wang
Front Pharmacol, 2020-05-19;11(0):700.
Species: Human
Sample Types: Tissue Homogenates
-
Glioblastoma Cell Resistance to EGFR and MET Inhibition Can Be Overcome via Blockade of FGFR-SPRY2 Bypass Signaling
Authors: EK Day, NG Sosale, A Xiao, Q Zhong, B Purow, MJ Lazzara
Cell Rep, 2020-03-10;30(10):3383-3396.e7.
Species: Human
Sample Types: Cell Culture Supernates
-
Individuality in FGF1 expression significantly influences platinum resistance and progression-free survival in ovarian cancer.
Br. J. Cancer, 2012-09-18;107(8):1327-36.
Species: Human
Sample Types: Cell Culture Supernates
-
Functional analysis of the chemokine receptor CCR3 on airway epithelial cells.
Authors: Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C
J. Immunol., 2006-09-01;177(5):3344-54.
Species: Human
Sample Types: Cell Culture Supernates
-
Humoral and cellular factors responsible for coronary collateral formation.
Authors: Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M
Am. J. Cardiol., 2006-08-31;98(9):1194-7.
Species: Human
Sample Types: Plasma
-
Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
Authors: Herrmann S, Green J, Weber T, Scharfenberger A
Bone, 2006-07-25;39(5):1156-63.
Species: Human
Sample Types: Tissue Homogenates
-
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005-11-01;175(9):6177-89.
Species: Human
Sample Types: Cell Culture Supernates
-
Alpha-tocopheryl succinate inhibits malignant mesothelioma by disrupting the fibroblast growth factor autocrine loop: mechanism and the role of oxidative stress.
Authors: Stapelberg M, Gellert N, Swettenham E, Tomasetti M, Witting PK, Procopio A, Neuzil J
J. Biol. Chem., 2005-05-05;280(27):25369-76.
Species: Human
Sample Types: Cell Culture Supernates
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQs
Loading...
Reviews for Human FGF acidic/FGF1 Quantikine ELISA Kit
Average Rating: 4.3 (Based on 3 Reviews)
Have you used Human FGF acidic/FGF1 Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Human FGF acidic/FGF1 Quantikine ELISA Kit
By Anonymous
on 09/24/2023
Sample Tested: Plasma,Urine
Plasma was measured at 10-fold and urine was measured at 4-fold dilution.
Human FGF acidic Quantikine ELISA Kit
By Anonymous
on 06/20/2016
Sample Tested: Muscle tissue
n/a
Human FGF acidic Quantikine ELISA Kit
By Anonymous
on 05/09/2016
Sample Tested: bone putty
