Human FGF basic/FGF2/bFGF Antibody

Catalog #: MAB2333 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2333-100
MAB2333-SP

Detection of FGF basic/FGF2/bFGF in A172 cells (positive) and Daudi cells (negative).

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Human FGF basic/FGF2/bFGF Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF basic/FGF2/bFGF in direct ELISA

Source

Monoclonal Mouse IgG_2A Clone # 10044

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived human FGF basic/FGF2/bFGF
Accession # P09038

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-25 µg/mL

Immersion fixed A172 human glioblastoma cells (positive) and Daudi human Burkitt's lymphoma cells (negative).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of FGF basic/FGF2/bFGF in A172 cells (positive) and Daudi cells (negative). FGF basic/FGF2/bFGF was detected in immersion fixed A172 human glioblastoma cells (positive) and absent in Daudi human Burkitt's lymphoma cells (negative) using Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2333) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic, also known as FGF-2 and HBGF-2, is a member of the FGF family of mitogenic proteins. FGF basic is widely expressed and affects diverse aspects of embryonic development by regulating cell proliferation, differentiation, and survival. Various N-terminal extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. Mature human FGF basic shares greater than 96% aa sequence identity with bovine, mouse, and rat FGF basic.

Long Name

Fibroblast Growth Factor basic

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Alternate Names

basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin