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Human FGF basic/FGF2/bFGF Antibody

Catalog #: MAB233
5 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB233-SP
MAB233-500
MAB233-100
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF basic/FGF2/bFGF Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human and bovine FGF basic/FGF2/bFGF. In capture ELISAs, shows approximately 0.2% cross-reactivity with bovine FGF acidic, and no cross-reactivity with recombinant human (rh) FGF acidic, rhFGF-4, rhFGF-6, or rhFGF-7.
Source
Monoclonal Mouse IgG2B Clone # 10060
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human FGF basic/FGF2/bFGF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Human FGF basic/FGF2/bFGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human FGF basic/FGF2/bFGF Biotinylated Antibody (Catalog # BAM233)

Standard: Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein (Catalog # 233-FB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic, also known as FGF2, is a member of the FGF family of growth factors.

Long Name
Fibroblast Growth Factor basic
Entrez Gene IDs
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin

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Citations for Human FGF basic/FGF2/bFGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons
    Authors: MEIQUN SUN, HONGLI LIU, SHENGPING MIN, HONGTAO WANG, XIAOJING WANG
    Biomedical Reports
  2. A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy
    Authors: J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau
    Nature Communications, 2021-02-17;12(1):1087.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  3. Heparan sulfate functions are altered in the osteoarthritic cartilage
    Authors: S Shamdani, S Chantepie, C Flageollet, N Henni-Cheb, Y Jouan, F Eymard, E Hay, M Cohen-Sola, D Papy-Garci, X Chevalier, P Albanese
    Arthritis Research & Therapy, 2020-12-07;22(1):283.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Capture
  4. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  5. Functional and structural remodeling of the myocardial microvasculature in early experimental hypertension.
    Authors: Rodriguez-Porcel M, Zhu XY, Chade AR, Amores-Arriaga B, Caplice NM, Ritman EL, Lerman A, Lerman LO
    Am. J. Physiol. Heart Circ. Physiol., 2005-10-07;290(3):H978-84.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

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Reviews for Human FGF basic/FGF2/bFGF Antibody

Average Rating: 4 (Based on 1 Review)

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Human FGF basic Antibody
By Anonymous on 11/16/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Together with BAM233 and ELISA was developed using this antibody as the capture molecule.

ELISA Human FGF basic Antibody MAB233