Human FGF basic/FGF2/bFGF Antibody

Catalog #: MAB2332 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2332-100
MAB2332-SP

Detection of FGF basic/FGF2/bFGF in Liver.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF basic/FGF2/bFGF Antibody Summary

Species Reactivity

Human

Specificity

Detects Human FGF basic/FGF2/bFGF in direct ELISA.

Source

Monoclonal Rat IgG_2A Clone # 954824

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived human FGF basic/FGF2/bFGF
Pro143-Ser288
Accession # P09038

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Liver

Immunocytochemistry

8-25 µg/mL

Immersion fixed A172 human glioblastoma cell line (Positive) & Daudi human Burkitt's lymphoma cell line (Negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of FGF basic/FGF2/bFGF in Liver. FGF basic/FGF2/bFGF was detected in immersion fixed paraffin-embedded sections of Liver using Rat Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2332) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry

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Detection of FGF basic/FGF2/bFGF in A172 (Positive) & Daudi (Negative). FGF basic/FGF2/bFGF was detected in immersion fixed A172 human glioblastoma cell line (Positive) & Daudi human Burkitt's lymphoma cell line (Negative) using Rat Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2332) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic (also known as FGF-2 and HBGF-2) is a member of the FGF superfamily of mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic are unique from other members of the family in that they lack classical secretory signal peptides. However, they are both readily secreted from cells by an alternative secretory pathway involving direct translocation and aided by several chaperones. FGF acidic (FGF-1) and FGF basic (FGF-2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. The full length human FGF basic protein is 288 amino acids, but there are multiple start sites which produce various shorter forms. Further adding to the complexity, a variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. FGF basic has been isolated from a number of sources, including neural tissue, adrenal cortex, pituitary gland, corpus luteum, and placenta. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is required for FGF binding with high affinity to FGF receptors. FGF basic stimulates proliferation of all cells of mesodermal origin as well as many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic also induces neuronal differentiation, survival, and regeneration, and modulates embryonic development and differentiation. These observed in vitro functions suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may also participate in the development of several pathological conditions resulting from excessive cell proliferation and/or angiogenesis.

References

Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506.
Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.

Long Name

Fibroblast Growth Factor basic

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Alternate Names

basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin