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Human FGF-BP DuoSet ELISA

Catalog #: DY1593-05
1 Citation 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY1593-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human FGF-BP ELISA Standard Curve
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human FGF-BP DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
23.4 - 1,500 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human FGF-BP. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Human FGF-BP ELISA Standard Curve View Larger

Human FGF-BP ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-BP

FGF binding protein (FGF-BP) was originally identified as a 17 kDa low-affinity heparin-binding protein in the conditioned medium of human A431 epidermoid carcinoma cells that bind FGF acidic and basic in a non-covalent, reversible manner. Rat FGF-BP cDNA encodes a 238 amino acid residue (aa) precursor protein with a 33 aa signal peptide. All FGF-BPs share ten conserved cysteine residues which form five intracellular disufide bridges.

Long Name:
Fibroblast Growth Factor Binding Protein
Entrez Gene IDs:
9982 (Human); 14181 (Mouse); 64535 (Rat)
Alternate Names:
17 kDa heparin-binding growth factor-binding protein; FGFBP; FGF-BP; FGFBP1; FGF-BP1; FGFBP-1; FGFBPFGF-binding protein 1; fibroblast growth factor binding protein 1; fibroblast growth factor-binding protein 1; HBp17; HBP1717 kDa HBGF-binding protein; heparin-binding growth factor binding protein

Citation for Human FGF-BP DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Poly-L-arginine promotes asthma angiogenesis through induction of FGFBP1 in airway epithelial cells via activation of the mTORC1-STAT3 pathway
    Authors: X Chen, M Miao, M Zhou, J Chen, D Li, L Zhang, A Sun, M Guan, Z Wang, P Liu, S Zhang, X Zha, X Fan
    Cell Death & Disease, 2021-08-02;12(8):761.
    Species: Human
    Sample Types: Serum

FAQs

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Reviews for Human FGF-BP DuoSet ELISA

Average Rating: 3.5 (Based on 2 Reviews)

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Human FGF-BP DuoSet ELISA
By Anonymous on 05/16/2022
Sample Tested: Serum and Plasma

We used this kit to quantify FGF-BP in human serum and plasma, need more serum/plasma sample.

Human FGF-BP DuoSet ELISA DY1593-05
Human FGF-BP DuoSet ELISA
By Jeff Pimentel on 11/02/2018
Sample Tested: Serum