Human FOLR1 APC-conjugated Antibody

Catalog #: FAB5646A Datasheet / COA / SDS

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FAB5646A

Detection of FOLR1 in MCF-7 Human Cell Line by Flow Cytometry.

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All FOLR1 Antibodies

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Human FOLR1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 548908

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
Arg25-Met233
Accession # P15328

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

MCF-7 human breast cancer cell line; HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of FOLR1 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 APC-conjugated Monoclonal Antibody (Catalog # FAB5646A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of FOLR1 in HeLa cells by Flow Cytometry HeLa cells were stained with Mouse Anti-Human FOLR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB5646A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).

References

Kelemen, L.E. (2006) Int. J. Cancer 119:243.
Fowler, B. et al. (2001) Kidney Int. 59:S-221.
Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
Ross, J.F. et al. (1994) Cancer 73:2432.
Parker, N. et al. (2005) Anal. Biochem. 338:284.
Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Long Name

Folate Receptor 1

Entrez Gene IDs

2348 (Human); 14275 (Mouse); 171049 (Rat)

Alternate Names

Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18