Human FOLR1 (Research Grade Mirvetuximab Biosimilar) Antibody

Catalog #: MAB11490 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB11490-100
MAB11490-SP

2 Images

All FOLR1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FOLR1 (Research Grade Mirvetuximab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects human FOLR1 ELISAs.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu218

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
Arg25-Met233
Accession # P15328

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

Thisantibody functions as an ELISA detection antibody for the specific antigen indirect ELISA. Colorimetric detection is performed after addition of a suitablesubstrate.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

View Larger

Human FOLR1 ELISA Standard Curve Direct ELISA binding curve demonstrating the recognition of Human Anti-Human FOLR1 (Research Grade Mirvetuximab Biosimilar) Monoclonal Antibody (Catalog # MAB11490) to FOLR1. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A goat anti-human HRP conjugate was used for detection.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).

References

Kelemen, L.E. (2006) Int. J. Cancer 119:243.
Fowler, B. et al. (2001) Kidney Int. 59:S-221.
Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
Ross, J.F. et al. (1994) Cancer 73:2432.
Parker, N. et al. (2005) Anal. Biochem. 338:284.
Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Long Name

Folate Receptor 1

Entrez Gene IDs

2348 (Human); 14275 (Mouse); 171049 (Rat)

Alternate Names

Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18