Human FOLR2 Biotinylated Antibody

Catalog #: BAF5697 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF5697

All FOLR2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FOLR2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FOLR2 in Western blots. In Western blots, less than 10% cross-reactivity with recombinant human (rh) FOLR1 and rhFOLR3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FOLR2
Gln22-His228
Accession # P14207

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human FOLR2 (Catalog # 5697-FR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR2

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34⁺ hematopoietic progenitor cells (4‑6). It is up‑regulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also up‑regulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8‑10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).

References

Fowler, B. et al. (2001) Kidney Int. 59:S-221.
Kelemen, L.E. (2006) Int. J. Cancer 119:243.
Shen, F. et al. (1994) Biochemistry 33:1209.
Ratnam, M. et al. (1989) Biochemistry 28:8249.
Ross, J.F. et al. (1999) Cancer 85:348.
Reddy, J.A. et al. (1999) Blood 93:3940.
Ross, J.F. et al. (1994) Cancer 73:2432.
Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub.
Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

Long Name

Folate Receptor 2

Entrez Gene IDs

2350 (Human); 14276 (Mouse); 293154 (Rat)

Alternate Names

beta-HFR; FBP; FBP/PL-1; folate receptor 2 (fetal); Folate receptor 2; folate receptor beta; folate receptor, beta; Folate receptor, fetal/placental; folate-binding protein, fetal/placental; FOLR2; FR-beta; FR-P3; Placental folate-binding protein