Human FOLR4 Antibody

Catalog #: AF6328 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6328
AF6328-SP

Detection of Human FOLR4 by Western Blot.

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All FOLR4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FOLR4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FOLR4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FOLR1, rhFOLR2, and rhFOLR3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FOLR4
Glu134-Asn210
Accession # NP_001073955

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FOLR4 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FOLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6328) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR4

FOLR4 (Folate receptor 4; also FR4 and folate receptor δ and folate binding protein 3) is a 34‑36 kDa member of the folate receptor family of molecules. Based on mouse, it appears to be a marker for subsets of T cells. In particular, and in conjunction with CD25, FR4^hiCD25^hi expression defines T reg cells, FR4^loCD25⁺ expression pattern identifies effector memory T cells, and a FR4^hiCD25^- combination characterizes central memory T cells. Mature human FOLR4 is 225 amino acids (aa) in length (aa 20‑244). It contains virtually no identifiable motifs. There is one potential splice variant that shows a five aa substitution for aa 111‑116. Over aa 134‑220, human FOLR4 shares 72% aa identity with mouse FOLR4.

Long Name

Folate Receptor 4

Entrez Gene IDs

390243 (Human); 64931 (Mouse); 690037 (Rat)

Alternate Names

folate receptor 4 (delta) homolog (mouse); Folbp3; FOLR4; FR-delta; LOC390243; probable folate receptor delta