Human Galectin-3 Biotinylated Antibody Summary
Ala2-Ile250
Accession # Q6IBA7
Customers also Viewed
Applications
Human Galectin-3 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-3
The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).
Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).
- Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
- Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
- Hughes, R.C. (2001) Biochimie 83:667.
- R&D Systems Cytokine Bulletin; Summer 2002.
- Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.
Product Datasheets
Citations for Human Galectin-3 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
Filter your results:
Filter by:
-
Chemically modified, non-anticoagulant heparin derivatives are potent galectin-3 binding inhibitors and inhibit circulating galectin-3-promoted metastasis
Authors: Carrie A. Duckworth, Scott E. Guimond, Paulina Sindrewicz, Ashley J. Hughes, Neil S. French, Lu-Yun Lian et al.
Oncotarget
-
Sequence‐Defined Heteromultivalent Precision Glycomacromolecules Bearing Sulfonated/Sulfated Nonglycosidic Moieties Preferentially Bind Galectin‐3 and Delay Wound Healing of a Galectin‐3 Positive Tumor Cell Line in an In Vitro Wound Scratch Assay
Authors: Tanja Freichel, Viktoria Heine, Dominic Laaf, Eleanor E. Mackintosh, Sophia Sarafova, Lothar Elling et al.
Macromolecular Bioscience
-
C1GalT1 expression reciprocally controls tumour cell-cell and tumour-macrophage interactions mediated by galectin-3 and MGL with double impact on cancer development and progression
Authors: Wan, Y;Adair, K;Herrmann, A;Shan, X;Xia, L;Duckworth, CA;Yu, LG;
Cell death & disease
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates
Authors: HM Hsu, YY Yang, YH Huang, CH Chu, TJ Tu, YT Wu, CJ Chiang, SB Yang, DK Hsu, FT Liu, JH Tai
PloS Neglected Tropical Diseases, 2023-01-03;17(1):e0011016.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Detection -
Galectin-3 deficiency in pregnancy increases the risk of fetal growth restriction (FGR) via placental insufficiency
Authors: N Freitag, I Tirado-Gon, G Barrientos, KL Powell, P Boehm-Stur, SP Koch, K Hecher, AC Staff, PC Arck, A Diemert, SM Blois
Cell Death Dis, 2020-07-23;11(7):560.
Species: Human
Sample Types: Serum
Applications: ELISA Detection -
Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners
Authors: E Gallardo-V, L Ruiz-Llore, J Casado-Vel, MJ Ruiz-Rodrí, N López-Andr, AK Pattnaik, M Quintanill, C Bernabeu
Cells, 2019-09-13;8(9):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mesenchymal stem cell-mediated T cell suppression occurs through secreted galectins.
Authors: Sioud M, Mobergslien A, Boudabous A, Floisand Y
Int. J. Oncol., 2010-12-09;38(2):385-90.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Galectin-Levels Are Elevated in Infants Born Preterm Due to Amniotic Infection and Rapidly Decline in the Neonatal Period
Authors: Kirstin Faust, Nancy Freitag, Gabriela Barrientos, Christoph Hartel, Sandra M. Blois
Frontiers in Immunology
-
Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression
Authors: Yaoyu Pang, Ellen Maxwell, Paulina Sindrewicz-Goral, Andrew Shapanis, Shun Li, Mark Morgan et al.
Biomolecules
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Staining Reagents
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Human Galectin-3 Biotinylated Antibody
Average Rating: 4.5 (Based on 2 Reviews)
Have you used Human Galectin-3 Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Specificity: Specific
Sensitivity: Sensitive
Buffer: Milk BSA Milk
Dilution: 1:1000
This antibody was first tested on tissues before the mouse IgG monoclonal version, the staining is diffuse and not as good as the other clone.
Specificity: Reasonably specific
Sensitivity: Not sensitive
Buffer: PBS
Dilution: 1:500
