Human gamma-Synuclein Antibody

Catalog #: MAB5745R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB5745R-SP
MAB5745R-500
MAB5745R-01M
MAB5745R-100

Detection of Human gamma -Synuclein by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human gamma-Synuclein Antibody Summary

Species Reactivity

Human

Specificity

Detects human gamma -Synuclein in direct ELISAs and Western blots.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 514304R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human gamma -Synuclein
Asn47-Glu120
Accession # Q6FHG5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

1-25 µg/mL

See below

Immunocytochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human gamma -Synuclein by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Recombinant Mouse Anti-Human gamma-Synuclein Monoclonal Antibody (Catalog # MAB5745R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for gamma-Synuclein at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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gamma -Synuclein in MBA‑MB‑468 Human Cell Line. gamma-Synuclein was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Recombinant Mouse Anti-Human gamma-Synuclein Monoclonal Antibody (Catalog # MAB5745R) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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gamma -Synuclein in Human Brain. gamma-Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Recombinant Mouse Anti-Human gamma-Synuclein Monoclonal Antibody (Catalog # MAB5745R) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gamma-Synuclein

gamma -Synuclein (SNCG), also called persyn or synoretin, is a 17 kDa, 127 amino acid (aa) cytoplasmic phosphoprotein. It is a member of a family of small, highly conserved synuclein proteins localized predominantly in presynaptic nerve terminals in the brain. SNCG and other synucleins are implicated in neurodegenerative diseases including Parkinson's disease. SNCG expression is elevated in many cancers and is implicated in tumor progression, invasion and proliferation. Within the region used as an immunogen, human SNCG shares 82% and 78% aa identity with mouse and rat SNCG, respectively.

Entrez Gene IDs

6623 (Human)

Alternate Names

BCSG1; BCSG1PERSYN; Breast cancer-specific gene 1 protein; gamma-Synuclein; persyn; PRSN; SNCG; SRPRSN; Synoretin; synuclein, gamma (breast cancer-specific protein 1); Synuclein-gamma