Human Gas1 Antibody

Catalog #: MAB2636
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2636
MAB2636-SP

All Gas1 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Gas1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Gas1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) Gas1, recombinant human (rh) Gas6, rhArtemin, rhNeurturin, rhRet, or rhShh N‑terminal domain. In Western blots, this antibody does not cross-react with rmGas6.

Source

Monoclonal Rat IgG_2A Clone # 309325

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Gas1
Leu40-Ser318
Accession # P54826

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Gas1 (Catalog # 2636-GS)
under non-reducing conditions only

Human Gas1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Gas1 Biotinylated Antibody (Catalog # BAF2636)

Standard: Recombinant Human Gas1 Protein, CF (Catalog # 2636-GS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gas1

Gas1 (Growth Arrest Specific 1) is one of six structurally unrelated proteins that were identified by their increased expression in growth-arrested cells relative to actively proliferating cells (1, 2). Following mitogenic stimulation, Gas1 expression is transcriptionally suppressed by c-Myc as cells transit from G₀ to G₁ phases of the cell cycle (3, 4). Overexpression of Gas1 prevents S phase entry and DNA synthesis (5). Gas1-mediated blockade of the cell cycle is p53-dependent but does not require the transactivating domain of p53 (6). The human Gas1 cDNA encodes a 345 amino acid (aa) precursor that includes a 39 aa signal sequence, a 279 aa mature protein, and a 27 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-rich regions as well as an RGD sequence (5). Mature human and mouse Gas1 share 85% aa sequence identity. Human Gas1 is a 40 kDa GPI-linked glycoprotein that is uniformly distributed on the cell surface (7). In contact-inhibited vascular endothelial cells, Gas1 is induced by VE-Cadherin and VEGF expression and mediates the anti-apoptotic effect of VEGF (8). In contrast, Gas1 is induced in hippocampal neurons after NMDA exposure but functions as a pro-apoptotic effector of NMDA-mediated excitotoxicity (9). Gas1 exhibits a range of developmental actions including either promoting or inhibiting growth and differentiation of somite, limb, cerebellar, and eye tissues (10 - 14). Gas1 contributes to the antagonistic effect of Wnt proteins toward Shh function by binding the N-terminal region of Shh (11). The dependence of Gas1 function on the cellular context has been addressed by suggesting that Gas1 could function as a co-receptor for GDNF family ligands (15). This speculation is supported by R&D Systems data which demonstrate direct binding of Gas1 to Artemin and Neurturin.

References

Schneider, C. et al. (1988) Cell 54:787.
Mullor, J.L. and A.R. Altaba (2002) BioEssays 24:22.
Del Sal, G. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1848.
Lee, T.C. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12886.
Del Sal, G. et al. (1992) Cell 70:595.
Del Sal, G. et al. (1995) Mol. Cell. Biol. 15:7152.
Stebel, M. et al. (2000) FEBS Lett. 481:152.
Spagnuolo, R. et al. (2004) Blood 103:3005.
Mellstrom, B. et al. (2002) Mol. Cell Neurosci. 19:417.
Lee, K.K.H. et al. (2001) Dev. Biol. 234:188.
Lee, C.S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11347.
Liu, Y. et al. (2002) Development 129:5289.
Liu, Y. et al. (2001) Dev. Biol. 236:30.
Lee, C.S. et al. (2001) Dev. Biol. 236:17.
Schueler-Furman, O. et al. (2006) Trends Pharmacol. Sci. 27:72.

Long Name

Growth-arrest-specific Protein 1

Entrez Gene IDs

2619 (Human); 14451 (Mouse)

Alternate Names

Gas1; GAS-1; growth arrest-specific 1; Growth arrest-specific gene-1; growth arrest-specific protein 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Gas1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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