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Human GATA-4 Antibody

Catalog #: AF2606
24 Citations 2 Reviews Datasheet / COA / SDS

Discontinued Product

AF2606 has been discontinued.
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Detection of Human GATA‑4 by Western Blot.
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Product Details
Citations (24)
FAQs
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GATA-4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GATA‑4 antibody by Western Blot. View Larger

Detection of Human GATA‑4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GATA-4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of GATA-4-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of GATA‑4-regulated Genes by Chromatin Immunoprecipitation. KATO-III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA-4/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human GATA-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Themucin4promoter was detected by standard PCR.

Immunocytochemistry GATA-4 antibody in Differentiated Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

GATA‑4 in Differentiated Human Embryonic Stem Cells. GATA‑4 was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry GATA-4 antibody in Differentiated Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

GATA‑4 in Differentiated Human Embryonic Stem Cells. GATA‑4 was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-4

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Entrez Gene IDs
2626 (Human)
Alternate Names
GATA binding protein 4; GATA4; GATA-4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; transcription factor GATA-4

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Citations for Human GATA-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Generation of TWO G51D SNCA missense mutation iPSC lines (CRICKi011-A, CRICKi012-A) from two individuals at risk of Parkinson's disease
    Authors: Devito, LG;Zanjani, ZS;Evans, JR;Scardamaglia, A;Houlden, H;Gandhi, S;Healy, L;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Paracrine interactions through FGFR1 and FGFR2 receptors regulate the development of preimplantation mouse chimaeric embryo
    Authors: K Krawczyk, K Wilczak, K Szczepa?sk, M Maleszewsk, A Suwi?ska
    Oncogene, 2022-11-16;12(11):220193.
    Species: Mouse
    Sample Types: Embryos
    Applications: IHC
  3. Generation of FOUR iPSC lines (CRICKi004-A; CRICKi005-A; CRICKi006-A, CRICKi007-A) from Spinal muscle atrophy patients with lower extremity dominant (SMALED) phenotype
    Authors: LG Devito, F Cooper, I D'Angelo, J Smith, L Healy
    Stem Cell Research, 2022-10-28;65(0):102954.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
    Authors: K Krawczyk, E Kosyl, K Cz??cik-?y, T Wyszomirsk, M Maleszewsk
    Scientific Reports, 2021-11-02;11(1):21422.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  5. Generation of an iPSC line (CRICKi001-A) from an individual with a germline SMARCA4 missense mutation and autism spectrum disorder
    Authors: LG Devito, L Healy, S Mohammed, F Guillemot, C Dias
    Stem Cell Research, 2021-03-20;53(0):102304.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  6. GATA4 blocks squamous epithelial cell gene expression in human esophageal squamous cells
    Authors: R Stavniichu, A DeLaForest, CA Thompson, J Miller, RF Souza, MA Battle
    Scientific Reports, 2021-02-05;11(1):3206.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  7. Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats
    Authors: P Khurana, N Kolundzic, M Rogar, C Hobbs, XFCC Wong, JEA Common, D Ilic, M Liovic
    Stem Cell Res, 2020-04-24;45(0):101827.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Pre-marked chromatin and transcription factor co-binding shape the pioneering activity of Foxa2
    Authors: FM Cernilogar, S Hasenöder, Z Wang, K Scheibner, I Burtscher, M Sterr, P Smialowski, S Groh, IM Evenroed, GD Gilfillan, H Lickert, G Schotta
    Nucleic Acids Res., 2019-09-26;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  9. FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation
    Authors: K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu
    Cell Rep, 2019-07-09;28(2):382-393.e7.
    Species: Human
    Sample Types: Chromatin
    Applications: Immunoprecipitation
  10. Induced pluripotent stem cell line heterozygous for p.R2447X mutation in filaggrin: KCLi002-A
    Authors: N Kolundzic, P Khurana, L Devito, M Donne, C Hobbs, J Jeriha, XFCC Wong, JAE Common, EB Lane, S Dubrac, R Gruber, M Schmuth, TM Mauro, D Ilic
    Stem Cell Res, 2019-05-13;38(0):101462.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Induced pluripotent stem cell (iPSC) line from an epidermolysis bullosa simplex patient heterozygous for keratin 5 E475G mutation and with the Dowling Meara phenotype
    Authors: N Kolundzic, P Khurana, C Hobbs, M Rogar, S Ropret, H Törmä, D Ilic, M Liovic
    Stem Cell Res, 2019-03-27;37(0):101424.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A
    Authors: L Devito, M Donne, N Kolundzic, P Khurana, C Hobbs, G Kaddour, S Dubrac, R Gruber, M Schmuth, T Mauro, D Ilic
    Stem Cell Res, 2018-07-25;31(0):122-126.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017-04-04;19(1):20-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Inhibiting Extracellular Vesicle Release from Human Cardiosphere Derived Cells with Lentiviral Knockdown of nSMase2 Differentially Effects Proliferation and Apoptosis in Cardiomyocytes, Fibroblasts and Endothelial Cells In Vitro
    PLoS ONE, 2016-11-02;11(11):e0165926.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  15. Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.
    Authors: Nagaoka M, Kobayashi M, Kawai C, Mallanna S, Duncan S
    PLoS ONE, 2015-08-26;10(8):e0136350.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  16. HEB associates with PRC2 and SMAD2/3 to regulate developmental fates.
    Authors: Yoon, Se-Jin, Foley, Joseph W, Baker, Julie C
    Nat Commun, 2015-03-16;6(0):6546.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta.
    Authors: Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar L, Pereira L
    J Virol, 2015-03-04;89(9):5134-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  18. A thyroid hormone receptor/KLF9 axis in human hepatocytes and pluripotent stem cells.
    Authors: Cvoro A, Devito L, Milton F, Noli L, Zhang A, Filippi C, Sakai K, Suh J, H Sieglaff D, Dhawan A, Sakai T, Ilic D, Webb P
    Stem Cells, 2015-02-01;33(2):416-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. GATA family members as inducers for cellular reprogramming to pluripotency.
    Authors: Shu, Jian, Zhang, Ke, Zhang, Minjie, Yao, Anzhi, Shao, Sida, Du, Fengxia, Yang, Caiyun, Chen, Wenhan, Wu, Chen, Yang, Weifeng, Sun, Yingli, Deng, Hongkui
    Cell Res, 2015-01-16;25(2):169-80.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  20. Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.
    Authors: Villa-Diaz L, Kim J, Lahann J, Krebsbach P
    Stem Cells Transl Med, 2014-10-13;3(12):1410-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Serine 105 phosphorylation of transcription factor GATA4 is necessary for stress-induced cardiac hypertrophy in vivo.
    Authors: van Berlo JH, Elrod JW, Aronow BJ
    Proc. Natl. Acad. Sci. U.S.A., 2011-07-11;108(30):12331-6.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  22. TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal.
    Authors: Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL
    Nat. Cell Biol., 2008-06-22;10(7):837-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
    Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
    Biotechnol. Bioeng., 2007-08-15;97(6):1617-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. The human mucin MUC4 is transcriptionally regulated by caudal-related homeobox, hepatocyte nuclear factors, forkhead box A, and GATA endodermal transcription factors in epithelial cancer cells.
    Authors: Jonckheere N, Vincent A, Perrais M, Ducourouble MP, Male AK, Aubert JP, Pigny P, Carraway KL, Freund JN, Renes IB, Van Seuningen I
    J. Biol. Chem., 2007-06-06;282(31):22638-50.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP

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Reviews for Human GATA-4 Antibody

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Human GATA-4 Antibody
By Megan Larson on 03/31/2017
Application: IHC Sample Tested: Pancreas tissue Species: Human and Mouse

(A) Human pancreas, 200X (B) Mouse pancreas, Gata4 wild-type, 200X (C) Mouse pancreas, Gata4 knock-out, 200X.

Immunohistochemistry Human GATA-4 Antibody AF2606
Human GATA-4 Antibody
By Megan Larson on 03/31/2017
Application: WB Sample Tested: Ovary tissue,PDAC Cell Lines Species: Mouse

75ug of protein from PDAC cell lines and 50 ug of protein from mouse ovary were loaded and incubated overnight at 1:1000 dilution with Gata4 antibody (AF2606). HRP-conjugated rabbit Anti-Goat IgG Secondary Antibody Rabbit was used . Vinculin was used as a loading control.

Western Blot Human GATA-4 Antibody AF2606