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Human GATA-6 Antibody

Catalog #: MAB1700
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1700
MAB1700-SP
GATA‑6 in HCT‑116 Human Cell Line.
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Product Details
Citations (5)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GATA-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) GATA-1 or rhGATA-2.
Source
Monoclonal Mouse IgG1 Clone # 222228
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GATA-6
Met1-Ala449
Accession # CAA64997
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human GATA‑6
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry GATA-6 antibody in HCT-116 Human Cell Line by Immunocytochemistry (ICC). View Larger

GATA‑6 in HCT‑116 Human Cell Line. GATA-6 was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Mouse Anti-Human GATA-6 Monoclonal Antibody (Catalog # MAB1700) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry GATA-6 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC). View Larger

GATA‑6 in HT‑29 Human Cell Line. GATA-6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human GATA-6 Monoclonal Antibody (Catalog # MAB1700) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-6

GATA-6 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-6 is expressed in visceral and parietal endoderm and other tissues. It interacts with several additional proteins to regulate gene expression in heart, lung, gut and gonads (1).

References
  1. Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.
Entrez Gene IDs
2627 (Human)
Alternate Names
GATA binding protein 6; GATA6; GATA-6; GATA-binding factor 6; GATA-binding protein 6; transcription factor GATA-6

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Citations for Human GATA-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Large-scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal cross talk
    Authors: Yu, L;Logsdon, D;Pinzon-Arteaga, CA;Duan, J;Ezashi, T;Wei, Y;Ribeiro Orsi, AE;Oura, S;Liu, L;Wang, L;Liu, K;Ding, X;Zhan, L;Zhang, J;Nahar, A;Stobbe, C;Katz-Jaffe, M;Schoolcraft, WB;Tan, T;Hon, GC;Yuan, Y;Wu, J;
    Cell stem cell
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
    Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
    International Journal of Molecular Sciences, 2022-06-30;23(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Self-organization of the human embryo in the absence of maternal tissues
    Nat. Cell Biol., 2016-05-04;18(6):700-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo.
    Authors: Morris SA, Teo RT, Li H, Robson P, Glover DM, Zernicka-Goetz M
    Proc. Natl. Acad. Sci. U.S.A., 2010-03-22;107(14):6364-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state.
    Authors: Lanner F, Lee KL, Sohl M, Holmborn K, Yang H, Wilbertz J, Poellinger L, Rossant J, Farnebo F
    Stem Cells, 2010-02-01;28(2):191-200.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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