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Human Glypican 4 Antibody

Catalog #: MAB9195
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9195-100
MAB9195-SP
Detection of Glypican 4 in Human TF-1 Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glypican 4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glypican 4 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 961609
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Glypican 4
Met1-Ser529
Accession # O75487
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB91951).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Glypican 4 antibody in Human TF-1 Cell Line antibody by Flow Cytometry. View Larger

Detection of Glypican 4 in Human TF-1 Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB9195, filled histogram) or Mouse IgG1 Isotype Control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Glypican 4 antibody in Human BG01V Cells antibody by Flow Cytometry. View Larger

Detection of Glypican 4 in Human BG01V Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB9195, filled histogram) or Mouse IgG1 Isotype Control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 4

Glypican 4, also known as K-Glypican, is an approximately 220 kDa GPI-anchored heparan sulfate proteoglycan with a 60 kDa protein core (1, 2). It is expressed in brain, kidney, adrenal gland, and fat tissue (1, 3) and binds to basic FGF (2). Mature human Glypican 4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat Glypican 4, respectively. An alternative splice isoform lacks the N-terminal 70 aa including the signal peptide. In the developing brain, Glypican 4 is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors (1, 2). It inhibits the dopaminergic differentiation of neurons (4). A 30 kDa cleaved form of Glypican 4 binds in cis to PTP sigma and contributes to excitatory synapse development and function (5). Glypican 4 is differentially expressed between adipose tissue depots (3). A soluble form can be released by adipocytes and circulates at elevated levels in obese patients with insulin resistance (3, 6). This form binds and enhances signaling through the Insulin R, and it also supports adipocyte differentiation (3). In zebrafish, Glypican 4 is required for cartilage formation and cardiomyocyte differentiation (7, 8).

References
  1. Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
  2. Hagihara, K. et al. (2000) Dev. Dyn. 219:353.
  3. Ussar, S. et al. (2012) Diabetes 61:2289.
  4. Fico, A. et al. (2014) J. Neurosci. 34:8318.
  5. Ko, J.S. et al. (2015) Proc. Natl. Acad. Sci. USA 112:1874.
  6. Zhu, H.J. et al. (2014) J. Endocrinol. Metab. 99:E2697.
  7. Sisson, B.E. et al. (2015) Mech. Dev. 138:279.
  8. Strate, I. et al. (2015) Development 142:1767.
Entrez Gene IDs
2239 (Human); 14735 (Mouse); 317322 (Rat)
Alternate Names
Glypican 4; glypican proteoglycan 4; glypican-4; GPC4; K-glypican; K-glypicandJ900E8.1 (glypican 4)

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Citations for Human Glypican 4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Surfaceome Profiling of Cell Lines and Patient-Derived Xenografts Confirm FGFR4, NCAM1, CD276, and Highlight AGRL2, JAM3, and L1CAM as Surface Targets for Rhabdomyosarcoma
    Authors: A Timpanaro, C Piccand, AC Uldry, PK Bode, D Dzhumashev, R Sala, M Heller, J Rössler, M Bernasconi
    International Journal of Molecular Sciences, 2023-01-30;24(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. PDX-derived organoids model in vivo drug response and secrete biomarkers
    Authors: L Huang, B Bockorny, I Paul, D Akshinthal, PO Frappart, O Gandarilla, A Bose, V Sanchez-Go, E Rouse, S Lehoux, N Pandell, C Lim, JG Clohessy, JE Grossman, RS Gonzalez, S Perea, G Daaboul, M Sawhney, SD Freedman, A Kleger, RD Cummings, A Emili, L Muthuswamy, M Hidalgo, S Muthuswamy
    JCI Insight, 2020-11-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot

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