Human HPRG Biotinylated Antibody

Catalog #: BAF1869 Datasheet / COA / SDS

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BAF1869

All HPRG Antibodies

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Human HPRG Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HPRG in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant mouse HPRG is observed and less than 1% cross-reactivity with recombinant human (rh) Cystatin A, rhCystatin B, rhCystatin C, rhCystatin D, rhCystatin F, and rhCystatin E/M is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HPRG
Val19-Lys525
Accession # P04196

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human HPRG (Catalog # 1869-HP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HPRG

Human histidine‑rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67‑75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N‑terminal cystatin‑like modules (aa 19‑254) and one His‑Pro‑rich region (aa 350‑497) that is flanked by two Pro‑rich segments (aa 276‑321 and 498‑525) (3, 4). The His‑Pro‑rich region contains 10 tandem repeats with an HHPHG motif, and the N‑ and C‑termini are linked by a disulfide bond (3, 5, 6). Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn⁺⁺‑dependent manner, it is converted to plasmin by tPA (7‑9). HPRG also shows antiangiogenic activity on endothelial cells (10).

References