Home / Hyaluronidase 1/HYAL1 / Human Hyaluronidase 1/HYAL1 DuoSet ELISA

Human Hyaluronidase 1/HYAL1 DuoSet ELISA

Catalog #: DY7358
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY7358
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human Hyaluronidase 1 / HYAL1 ELISA Standard Curve
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage

Human Hyaluronidase 1/HYAL1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Hyaluronidase 1 / HYAL1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Human Hyaluronidase 1 / HYAL1 ELISA Standard Curve View Larger

Human Hyaluronidase 1 / HYAL1 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Human Hyaluronidase 1/HYAL1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Targeting BET Proteins Decreases Hyaluronidase-1 in Pancreatic Cancer
    Authors: Kumar, K;Kanojia, D;Bentrem, DJ;Hwang, RF;Butchar, JP;Tridandapani, S;Munshi, HG;
    Cells
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Hyaluronidase-1-mediated glycocalyx impairment underlies endothelial abnormalities in polypoidal choroidal vasculopathy
    Authors: KX Wu, NJY Yeo, CY Ng, FWJ Chioh, Q Fan, X Tian, B Yang, G Narayanan, HM Tay, HW Hou, NR Dunn, X Su, CMG Cheung, C Cheung
    Bmc Biology, 2022-02-15;20(1):47.
    Species: Human
    Sample Types: Plasma
  3. Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner
    Authors: W Zhang, G Yin, H Zhao, H Ling, Z Xie, C Xiao, Y Chen, Y Lin, T Jiang, S Jin, J Wang, X Yang
    Cell Death & Disease, 2021-01-20;12(1):102.
    Species: Human
    Sample Types: Serum

FAQs

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