Human IGF-II/IGF2 Antibody

Detection of Human IGF-II/IGF2 by Western Blot. Western blot shows lysates of human liver tissue, Huh-7 human hepatoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB2921) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-II/IGF2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of IGF-II/IGF2 by Western Blot The MYOD1-mediated IGF2 gene expressions and cell viability. (A,B) mRNA and protein expression levels of MYOD1 in CEFs overexpressing distinct siRNAs. (C) Proliferation analysis of MYOD1-silenced CEF cells. CEF cells transfected with MYOD1 siRNAs were harvested at 48 hpt for CCK-8 assay. (D,E) RT-qPCR and western blot analysis of gga-miR-223 and IGF2 expressions in MYOD1-silenced CEF cells at 48 hpt. (F,G) Expression levels of gga-miR-223, MYOD1, and IGF2 proteins in CEFs overexpressing miR-M2-5p determined by RT-qPCR or western blot analysis. (H) Relative signal intensities in western blot analysis of the MYOD1 and IGF2 proteins in CEFs overexpressing miR-M2-5p. (I,J) Expression levels of gga-miR-223, MYOD1, and IGF2 proteins in GaHV-2-infected CEFs determined by RT-qPCR or western blot analysis. (K) Relative signal intensities in western blot analysis of the MYOD1 and IGF2 proteins in GaHV-2-infected CEFs. Numbers were normalized to the corresponding signal from the beta -actin bands. Error bars are derived from three independent replicates. Values of p indicated on columns were used for statistical analyses; ns, no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33224130), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IGF-II/IGF2 by Western Blot Proliferation and apoptosis of miR-M2-5p inhibitor-transfected MSB-1 cells. (A) RT-qPCR analysis of the relative expression levels of viral protein-coding or miRNA genes in MSB-1 cells at 24 hpt with miR-M2-5p inhibitor. (B,C) Cell viability and apoptosis of the MSB-1 cells transfected with miR-M2-5p inhibitor. MSB-1 cells transfected with miR-M2-5p inhibitor were harvested at 24 hpt for Annexin V-FITC and propidium iodide (PI) staining for apoptosis analysis using flow cytometry. CCK-8 assay was used to assess cell viability. (D) Western blot analysis of proteins associated with the cell proliferation or apoptosis in MSB-1 cells at 24 hpt. Numbers below the blots indicate relative band intensity normalized to beta -actin. Error bars are derived from three independent replicates. Values of p indicated on columns were used for statistical analyses; ns, no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33224130), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IGF-II/IGF2 by Western Blot The MYOD1-mediated IGF2 gene expressions and cell viability. (A,B) mRNA and protein expression levels of MYOD1 in CEFs overexpressing distinct siRNAs. (C) Proliferation analysis of MYOD1-silenced CEF cells. CEF cells transfected with MYOD1 siRNAs were harvested at 48 hpt for CCK-8 assay. (D,E) RT-qPCR and western blot analysis of gga-miR-223 and IGF2 expressions in MYOD1-silenced CEF cells at 48 hpt. (F,G) Expression levels of gga-miR-223, MYOD1, and IGF2 proteins in CEFs overexpressing miR-M2-5p determined by RT-qPCR or western blot analysis. (H) Relative signal intensities in western blot analysis of the MYOD1 and IGF2 proteins in CEFs overexpressing miR-M2-5p. (I,J) Expression levels of gga-miR-223, MYOD1, and IGF2 proteins in GaHV-2-infected CEFs determined by RT-qPCR or western blot analysis. (K) Relative signal intensities in western blot analysis of the MYOD1 and IGF2 proteins in GaHV-2-infected CEFs. Numbers were normalized to the corresponding signal from the beta -actin bands. Error bars are derived from three independent replicates. Values of p indicated on columns were used for statistical analyses; ns, no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33224130), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IGF-II/IGF2 by Western Blot The MYOD1-mediated IGF2 gene expressions and cell viability. (A,B) mRNA and protein expression levels of MYOD1 in CEFs overexpressing distinct siRNAs. (C) Proliferation analysis of MYOD1-silenced CEF cells. CEF cells transfected with MYOD1 siRNAs were harvested at 48 hpt for CCK-8 assay. (D,E) RT-qPCR and western blot analysis of gga-miR-223 and IGF2 expressions in MYOD1-silenced CEF cells at 48 hpt. (F,G) Expression levels of gga-miR-223, MYOD1, and IGF2 proteins in CEFs overexpressing miR-M2-5p determined by RT-qPCR or western blot analysis. (H) Relative signal intensities in western blot analysis of the MYOD1 and IGF2 proteins in CEFs overexpressing miR-M2-5p. (I,J) Expression levels of gga-miR-223, MYOD1, and IGF2 proteins in GaHV-2-infected CEFs determined by RT-qPCR or western blot analysis. (K) Relative signal intensities in western blot analysis of the MYOD1 and IGF2 proteins in GaHV-2-infected CEFs. Numbers were normalized to the corresponding signal from the beta -actin bands. Error bars are derived from three independent replicates. Values of p indicated on columns were used for statistical analyses; ns, no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33224130), licensed under a CC-BY license. Not internally tested by R&D Systems.