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Human IGFL-3 Antibody

Catalog #: MAB5970 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5970
MAB5970-SP
Detection of Human IGFL-3 by Western Blot.
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Human IGFL-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFL-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IGF‑I, rhIGF-II, rhIGFL-1, rhIGFL-4, and recombinant mouse IGFL-3 is observed.
Source
Monoclonal Mouse IgG2B Clone # 536825
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IGFL-3
Thr25-Pro125
Accession # Q6UXB1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IGFL-3 antibody by Western Blot. View Larger

Detection of Human IGFL-3 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGFL-3 Monoclonal Antibody (Catalog # MAB5970) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGFL-3 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFL-3

IGFL-3 (insulin-like growth factor-like 3) is one of four small (~11 kDa), probably secreted human IGFL family members. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family. Mature IGFL-3 is a 101 amino acid (aa) protein that shares 41% aa identity with the sole mouse IGFL. RT-PCR data indicates its expression within the cerebellum.

Long Name
IGF-like Family Member 3
Entrez Gene IDs
388555 (Human)
Alternate Names
IGFL3; IGFL-3; UNQ483

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