Human IKK beta Antibody

Name: Human IKK beta Antibody
Brand: R&D Systems
SKU: AF4535
Rating: 5 (1 reviews)

Catalog #: AF4535
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4535
AF4535-SP

Detection of Human IKK beta by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IKK beta Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human IKK beta in Western blots. In Western blots, this antibody does not cross-react with recombinant human IKK alpha, IKK gamma, or IKK epsilon.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IKK beta
Gly374-Ser756
Accession # O14920

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IKK beta by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human IKK beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4535) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IKK beta at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK beta

I kappa B kinase beta (IKK beta ) is also known as IKBKB and IKK2. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. Like IKK alpha, IKK beta contains kinase, leucine zipper, and helix‑loop‑helix domains. NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha /IKK beta heterodimers.

Long Name

IkB Kinase beta

Entrez Gene IDs

3551 (Human); 16150 (Mouse); 84351 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK2; IKK2FLJ38368; IKK-B; IKK-betaFLJ36218; IKKBFLJ40509; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; NFKBIKBFLJ33771; Nuclear factor NF-kappa-B inhibitor kinase beta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IKK beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Keap1 regulates inflammatory signaling in Mycobacterium avium-infected human macrophages.
Authors: Awuh J, Haug M, Mildenberger J, Marstad A, Do C, Louet C, Stenvik J, Steigedal M, Damas J, Halaas O, Flo T
Proc Natl Acad Sci U S A, 2015-07-20;112(31):E4272-80.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-kappaappa B signaling induced by tumor necrosis factor-alpha in human A549 lung epithelial cells.
Authors: Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
PLoS ONE, 2014-06-24;9(6):e100787.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot