Human IL-17E/IL-25 Antibody

Catalog #: MAB12582 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB12582-100
MAB12582-SP

Human IL‑17E/IL‑25 ELISA Standard Curve.

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All IL-17E/IL-25 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-17E/IL-25 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-17E/IL-25 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 878227

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL-17E/IL-25
Tyr33-Gly177
Accession # Q9H293

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IL‑17E/IL‑25 Monoclonal Antibody (Catalog # MAB12581).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-17E DuoSet ELISA Kit (Catalog # DY1258-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human IL‑17E/IL‑25 ELISA Standard Curve. Recombinant Human IL-17E/IL-25 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB12582) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB12581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17E/IL-25

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, and IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Human IL-17E cDNA encodes a 177 amino acid (aa) residues precursor protein with a putative 32 aa signal peptide (3). A second isoform of human IL-17E encoding a 161 aa precursor protein also exists (4). The two isoforms differ in their signal peptide sequences. Mature human IL-17E shares 76% aa sequence identity with mature mouse IL-17E. Human IL-17E also shares from 25-36% aa sequence identity with the other human IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland, spinal cord, and trachea (3). IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3), which is expressed in kidney and liver, and at lower levels in brain, testis, and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the pro-inflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

References