Human IL-1 beta /IL-1F2 APC-conjugated Antibody
Human IL-1 beta /IL-1F2 APC-conjugated Antibody Summary
Ala117-Ser269
Accession # P01584
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑1 beta /IL‑1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes either (A) untreated or (B) treated with 250 ng/mL LPS overnight were stained with Rabbit Anti-Human IL-1 beta /IL-1F2 APC-conjugated Monoclonal Antibody (Catalog # IC8406A) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC105A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of Human IL-1 beta/IL-1F2 by Flow Cytometry Knock-down of ATG4D by shRNA in UTSM cell line mimic UF phenotype. (a) Autophagy markers analysis by FACS with intracellular staining and mean fluorescence intensity MFI for LC3, and (b) by western blot for LC3 and P62. (c) Extracellular matrix markers analysis by western blot. (d) Endogenous quantification of pro-inflammatory cytokines expression was done by intracellular staining for TNF-alpha, IL-1 beta, TGF-beta and IL-10 using flow cytometry analysis. The expression level was represented as mean fluorescence intensity (MFI). Data from the histogram are shown as mean±S.D. and are representative of three independent experiments. *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/cddiscovery201741), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
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