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Human IL-1 beta /IL-1F2 Biotinylated Antibody

Catalog #: BAF201
29 Citations Datasheet / COA / SDS
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BAF201
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IL‑1 beta /IL‑1F2 in Human PBMCs.
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Human IL-1 beta /IL-1F2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 4% cross-reactivity with recombinant rat
IL‑1 beta is observed and less than 0.1% cross-reactivity with recombinant mouse IL‑1 beta and recombinant porcine IL‑1 beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-1 beta /IL-1F2
Ala117-Ser269
Accession # NP_000567
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB)
Immunocytochemistry
5-15 µg/mL
See below

Human IL-1 beta /IL-1F2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human IL‑1 beta /IL‑1F2 Antibody (Catalog # MAB601)

Capture Reagent: Human IL‑1 beta /IL‑1F2 Antibody (Catalog # MAB601R)

Standard: Recombinant Human IL-1 beta/IL-1F2 Protein (Catalog # 201-LB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL-1 beta /IL-1F2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-1 beta /IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry IL-1 beta /IL-1F2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-1 beta /IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Functional Detection of Human IL-1 beta/IL-1F2 by Functional View Larger

Detection of Human IL-1 beta/IL-1F2 by Functional Differences in Functional Profiles between Three Patients with Different Clinical Outcomesgp100 209–2M spots co-spotted with anti-IFN gamma, anti-TNF alpha, anti-IL1b, and anti-IL-6 are shown for three separate patient samples. Patient 10735, who remains free of disease at this writing, displays strong IFN gamma, TNF alpha, IL-1b, and IL-6 secretion. Patients 10710 and 10737 show strong IFN gamma secretion, but weak to no TNF alpha, IL-1b, and IL-6 secretion; both patients experienced disease recurrence soon after these samples were drawn. Note the diffuse pattern of IL-1b and IL-6 capture, which differs from the focal capture of IFN gamma and TNF alpha. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/16162034), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Citations for Human IL-1 beta /IL-1F2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Machine learning identifies molecular regulators and therapeutics for targeting SARS‐CoV2‐induced cytokine release
    Authors: Marina Chan, Siddharth Vijay, John McNevin, M Juliana McElrath, Eric C Holland, Taranjit S Gujral
    Molecular Systems Biology
  2. Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens
    Authors: Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al.
    Frontiers in Immunology
  3. Identification of Predictive Early Biomarkers for Sterile-SIRS after Cardiovascular Surgery.
    Authors: Stoppelkamp S, Veseli K, Stang K, Schlensak C, Wendel H, Walker T
    PLoS ONE, 2015-08-11;10(8):e0135527.
  4. The Global Phosphorylation Landscape of SARS-CoV-2 Infection
    Authors: Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al.
    Cell
  5. Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways
    Authors: Lai J, Wu D, Wu C et al.
    iScience
  6. Structural Modification of the Antidepressant Mianserin Suggests That Its Anti-inflammatory Activity May Be Independent of 5-Hydroxytryptamine Receptors
    Authors: Sandra Sacre, Albert Jaxa-Chamiec, Caroline M. R. Low, Giselle Chamberlain, Cathy Tralau-Stewart
    Frontiers in Immunology
  7. Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis
    Authors: Tomas Castro-Dopico, Thomas W. Dennison, John R. Ferdinand, Rebeccah J. Mathews, Aaron Fleming, Dean Clift et al.
    Immunity
  8. Differences in the effects of four TRPV1 channel antagonists on lipopolysaccharide-induced cytokine production and COX-2 expression in murine macrophages
    Authors: Y Ninomiya, SI Tanuma, M Tsukimoto
    Biochem. Biophys. Res. Commun, 2017-01-31;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. A20 restricts ubiquitination of pro-interleukin-1beta protein complexes and suppresses NLRP3 inflammasome activity.
    Authors: Duong B, Onizawa M, Oses-Prieto J, Advincula R, Burlingame A, Malynn B, Ma A
    Immunity, 2015-01-20;42(1):55-67.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon SJ, Moon S, Joo YB, Joo Y, Jeon CH, Jeon C, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 2011-09-01;26(9):1132-9.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  11. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009-10-07;183(9):5799-806.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  12. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  13. Endogenous IL-32 controls cytokine and HIV-1 production.
    Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
    J. Immunol., 2008-07-01;181(1):557-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  14. Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
    Authors: Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE
    AIDS Res. Hum. Retroviruses, 2008-05-01;24(5):667-71.
    Species: Human
    Sample Types: Vaginal Fluid
    Applications: ELISA Development
  15. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  16. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  17. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  18. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
    Authors: Emsley HC, Smith CJ, Gavin CM
    BMC Neurol, 2007-02-28;7(0):5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  19. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  20. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  21. HIV-1-infected and/or immune activated macrophages regulate astrocyte SDF-1 production through IL-1beta.
    Authors: Peng H, Erdmann N, Whitney N, Dou H, Gorantla S, Gendelman HE, Ghorpade A, Zheng J
    Glia, 2006-11-01;54(6):619-29.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  22. Changes in periodontal and rheumatological conditions after 2 years in patients with juvenile idiopathic arthritis.
    Authors: Miranda LA, Braga F, Fischer RG, Sztajnbok FR, Figueredo CM, Gustafsson A
    J. Periodontol., 2006-10-01;77(10):1695-700.
    Species: Human
    Sample Types:
    Applications: ELISA Development
  23. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Kiryushko D, Pavon L, Novitskaya V, Sandoval-Lopez G, Soroka V, Eugenia Hernandez M, Klingelhofer J, Loria F, Lukanidin E, Estrada I, Berezin V, Perez M, Bock E, Moreno J, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006-02-02;172(1):156-65.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  24. Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.
    Authors: Chen DS, Soen Y, Stuge TB
    PLoS Med., 2005-09-20;2(10):e265.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  25. Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation.
    Authors: Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M
    Eur. J. Neurosci., 2004-12-01;20(12):3222-32.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  26. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004-01-01;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  27. Offloading treatment is linked to activation of proinflammatory cytokines and start of bone repair and remodeling in Charcot arthropathy patients
    Authors: Agnetha Folestad, Martin Ålund, Susanne Asteberg, Jesper Fowelin, Ylva Aurell, Jan Göthlin et al.
    Journal of Foot and Ankle Research
  28. Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips
    Authors: Manfè Valentina, Fleckner Jan, Nørby Lisby Peder, Zhang Bo, Dai Hongjie, Keller Pernille
    BMC Biotechnology
  29. Antibody and DNA sensing pathways converge to activate the inflammasome during primary human macrophage infection
    Authors: Larisa I Labzin, Maria Bottermann, Pablo Rodriguez‐Silvestre, Stian Foss, Jan Terje Andersen, Marina Vaysburd et al.
    The EMBO Journal

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