Human IL-2 Quantikine HS ELISA Kit

Catalog #: HS200
7 Citations Datasheet / COA / SDS

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HS200

Control Products Available

QC238: Quantikine Immunoassay Control Set 933 for Human HS IL-2

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Citations (7)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-2 Quantikine HS ELISA Kit Summary

Assay Length

4.0 hours

Sample Type & Volume Required Per Well

Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)

Sensitivity

0.066 pg/mL

Assay Range

0.3 - 20 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IL-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine® HS Human IL-2 Immunoassay kit is a 4.0 hour solid phase ELISA designed to measure human IL-2 levels in serum and plasma. It contains
E. coli-expressed recombinant human IL-2 and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-2 accurately. Results obtained using natural human IL-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine® HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	1.89	5.6	11.3	1.89	5.59	11.4
Standard Deviation	0.04	0.13	0.225	0.133	0.327	0.787
CV%	2.1	2.3	2	7	5.8	6.9

Recovery

The recovery of natural human IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	91	84-96
Heparin Plasma (n=4)	88	79-95
Serum (n=4)	91	86-94

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of natural human IL-2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human IL-2 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a 15-18 kDa variably glycosylated alpha -helical polypeptide that is a member of the Common gamma Chain ( gamma c) cytokine family (1-4). It exists as a monomer and has a notably short half-life (< 30 minutes) (1). Human IL-2 is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 133 aa mature region (5, 6). The mature region is alpha -helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity (7). Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gamma δ T cells (8), activated conventional CD4+ and CD8+ T cells (1, 9), neurons (10, 11), microglia (12), and hematopoietic stem cells (13).

The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain (1, 3). IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gamma c to form the quaternary signaling complex (1, 14). In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gamma c also serves as a signaling receptor for IL-4, -7, -9, -15, and -21 (1, 3).

In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells. (1).

Long Name:

Interleukin 2

Entrez Gene IDs:

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names:

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-2 Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Characterizations of a neutralizing antibody broadly reactive to multiple gluten peptide:HLA-DQ2.5 complexes in the context of celiac disease
Authors: Okura, Y;Ikawa-Teranishi, Y;Mizoroki, A;Takahashi, N;Tsushima, T;Irie, M;Harfuddin, Z;Miura-Okuda, M;Ito, S;Nakamura, G;Takesue, H;Ozono, Y;Nishihara, M;Yamada, K;Gan, SW;Hayasaka, A;Ishii, S;Wakabayashi, T;Muraoka, M;Nagaya, N;Hino, H;Nemoto, T;Kuramochi, T;Torizawa, T;Shimada, H;Kitazawa, T;Okazaki, M;Nezu, J;Sollid, LM;Igawa, T;
Nature communications
Species: Human
Sample Types: Cell Culture Supernates
Inflammatory Markers and Risk of Heart Failure With Reduced to Preserved Ejection Fraction
Authors: Z Albar, M Albakri, J Hajjari, M Karnib, SE Janus, SG Al-Kindi
The American journal of cardiology, 2022-01-03;0(0):.
Species: Human
Sample Types: Serum
Comparative evaluation of inflammatory parameters and substitute insulin resistance indices in elderly women with and without type 2 diabetes mellitus
Authors: MM Leite, MT Dutra, MVG da Costa, SS Funghetto, AO Silva, LR de Lima, ICR da Silva, MR Mota, MM Stival
Experimental gerontology, 2021-05-03;150(0):111389.
Species: Human
Sample Types: Plasma
Changes in protein expression due to metformin treatment and hyperinsulinemia in a human endometrial cancer cell line
Authors: C Lange, A Machado We, R Schmidt, C Schroeder, T Strowitzki, A Germeyer
PLoS ONE, 2021-03-09;16(3):e0248103.
Species: Human
Sample Types: Cell Culture Supernates
New signatures of poor CD4 cell recovery after suppressive antiretroviral therapy in HIV-1-infected individuals: involvement of miR-192, IL-6, sCD14 and miR-144
Authors: F Hernández-, MJ Ruiz-de-Le, I Rosado-Sán, E Vázquez, M Leal, S Moreno, F Vidal, J Blanco, YM Pacheco, A Vallejo
Sci Rep, 2020-02-19;10(1):2937.
Species: Human
Sample Types: Plasma
Association between baseline pro-inflammatory cytokines and brain activation during social exclusion in patients with vulnerability to suicide and depressive disorder
Authors: I Conejero, I Jaussent, A Cazals, E Thouvenot, T Mura, E Le Bars, S Guillaume, S Squalli, P Courtet, E Olié
Psychoneuroendocrinology, 2018-10-04;99(0):236-242.
Species: Human
Sample Types: Serum
Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
Clin. Cancer Res., 2006-01-15;12(2):376-82.
Species: Human
Sample Types: Plasma