Human Importin alpha 2/KPNA2 Antibody

Catalog #: MAB62071 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB62071
MAB62071-SP

Importin alpha 2/KPNA2 in NTera‑2 Human Cell Line.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Importin alpha 2/KPNA2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Importin alpha 2/KPNA2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) KPNA1, rhKPNA3, rhKPNA4, rhKPNA5, or rhKPNB1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 682208

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Importin alpha 2/KPNA2
Ser2-Asn132
Accession # P52292

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Importin alpha 2/KPNA2 in NTera‑2 Human Cell Line. Importin a2/KPNA2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB62071) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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Importin alpha 2/KPNA2 in HeLa Human Cell Line. Importin alpha 2/KPNA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Importin alpha 2/KPNA2 Monoclonal Antibody (Catalog # MAB62071) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Importin alpha 2/KPNA2

Importin subunit alpha 2, also known as KPNA2 (Karyopherin subunit alpha 2), SRP1a, and RAG1, is a 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. Importin alpha 2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, Importin alpha 2 first binds to NBS1, and then to Importin beta 1, which mediates movement through the nuclear pore. Human Importin alpha 2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/Importin b domain (aa 2-60), ten Armadillo repeats that bind “cargo” (aa 71-496) and two intervening NLS binding sites. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. The aminoacid region 1-132 of human Importin alpha 2 shares a 94% homology with mouse Importin alpha 2.

Long Name

Karyopherin alpha 2

Entrez Gene IDs

3838 (Human); 16647 (Mouse); 85245 (Rat)

Alternate Names

Importin alpha 1; Importin alpha 2; importin-alpha-P1; IPOA1; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); Karyopherin subunit alpha-2; KPNA2; Pendulin; QIP2; QIP2importin alpha 2; RAG cohort 1; RAG cohort protein 1; RCH1; RCH1importin subunit alpha-2; SRP1; SRP1alpha; SRP1-alpha