Human Importin alpha 2/KPNA2 Antibody Summary
Ser2-Asp132
Accession # P52292
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Importin alpha 2/KPNA2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB6207) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Importin a2/KPNA2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Importin alpha 2/KPNA2 in HeLa Human Cell Line. Importin a2/KPNA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB6207) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Importin alpha 2/KPNA2
Importin alpha 2, also known as KPNA2 (Karyopherin subunit alpha 2), SRP1a, or RAG1, is a 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. Importin alpha 2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, Importin alpha 2 first binds to NBS1, and then to Importin beta 1, which mediates movement through the nuclear pore. Human Importin alpha 2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/Importin beta domain (aa 2-60), ten Armadillo repeats that bind “cargo” (aa 71-496) and two intervening NLS binding sites. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. Over aa 1-132, human Importin alpha 2 shares 94% aa identity with mouse Importin alpha 2.
Product Datasheets
Citations for Human Importin alpha 2/KPNA2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Mechanical force application to the nucleus regulates nucleocytoplasmic transport
Authors: I Andreu, I Granero-Mo, NR Chahare, K Clein, M Molina-Jor, AEM Beedle, A Elosegui-A, JF Abenza, L Rossetti, X Trepat, B Raveh, P Roca-Cusac
Nature Cell Biology, 2022-06-09;24(6):896-905.
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Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death
Authors: Gasset-Rosa F, Lu S, Yu H et al.
Neuron
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Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α
Authors: CC Chang, CJ Chen, C Grauffel, YC Pien, C Lim, SY Tsai, KC Hsia
Nat Commun, 2019-09-20;10(1):4307.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation -
Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-?/?1-Independent
PLoS ONE, 2016-08-31;11(8):e0162033.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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