Human Integrin beta 3/CD61 Antibody Summary
Gly27-Asp718
Accession # P05106
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Integrin beta 3/CD61 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Integrin beta 3/ CD61 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2266) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin beta 3/CD61 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Integrin beta 3/CD61 by Simple WesternTM. Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin beta 3/CD61 at approximately 116 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Integrin beta 3/CD61 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2266). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 3/CD61
Integrin beta 3, also called CD61, associates with the Integrin alpha IIb or alpha V subunits to form heterodimeric receptors for collagens, fibronectin, vitronectin, fibrinogen, Von willebrand factor or thrombospondin. alpha IIb/ beta 3, also known as GPIIb IIIa (Glycoprotein IIb-IIIa), is expressed on platelets. alpha V/ beta 3, also known as vitronectin receptor, is expressed on endothelial cells, some B cells, platelets and monocytes.
Product Datasheets
Citations for Human Integrin beta 3/CD61 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species
Authors: Tabebordbar M, Lagerborg KA, Stanton A et al.
Cell
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Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood
Authors: WM Dickerson, R Yu, HU Westergren, J Paraskos, P Schatz, A Tigerstrom, A Ekman, J Sánchez, J Cheng, L Li, EY Chan
PLoS ONE, 2021-08-26;16(8):e0256423.
Species: Human
Sample Types: Plasma
Applications: Interferent Testing -
Platelet-derived extracellular vesicles are increased in sera of Alzheimer's disease patients, as revealed by Tim4-based assays
Authors: H Odaka, K Hiemori, A Shimoda, K Akiyoshi, H Tateno
FEBS Open Bio, 2021-02-03;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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