Human ITM2C Antibody

Catalog #: AF4904 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4904
AF4904-SP

Detection of Human ITM2C by Western Blot.

1 Image

All ITM2C Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ITM2C Antibody Summary

Species Reactivity

Human

Specificity

Detects human ITM2C in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ITM2A and rhITM2B is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ITM2C
Ala86-Val267
Accession # Q9NQX7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human ITM2C by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ITM2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4904) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITM2C at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITM2C

ITM2C (Integral membrane protein 2C; also BRI3 and cerebral protein 14) is a 36‑38 kDa member of the BRI₃ family of proteins. It is found on neurons and appears to interact with APP, where ITM2C binding blocks proteolytic processing of APP, plus SCG10, where ITM2C binding blocks the microtubule-destabilizing activity of SCG10, thus inhibiting neurite outgrowth. Human ITM2C is a 267 amino acid (aa) type II transmembrane glycoprotein. Proteolytic cleavage by furin at Arg242Gly243 may create a truncated 34‑35 kDa ITM2C plus a C-terminal 4 kDa active peptide (aa 243‑267). The ECD (aa 76‑267) contains one BRICHOS domain (aa 136‑230). ITM2C may exist as a dimer. There are two splice variants, one that shows a deletion of aa 41‑87, and a second that shows a deletion of aa 151‑187. Over aa 86‑267, human ITM2C shares 96% aa identity with mouse ITM2C.

Long Name

Integral Membrane Protein 2C

Entrez Gene IDs

81618 (Human); 64294 (Mouse); 301575 (Rat)

Alternate Names

BRI3; BRI3E25C; BRICD2C; BRICHOS domain containing 2C; Cerebral protein 14; E25; hRPC.1050_D_4; integral membrane protein 2C; integral membrane protein 3; ITM2C; ITM3; Transmembrane protein BRI3