Human Jagged 1 Biotinylated Antibody

Catalog #: BAF1277
1 Citation Datasheet / COA / SDS

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BAF1277

All Jagged 1 Antibodies

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Human Jagged 1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Jagged 1 in Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant rat Jagged 1 is observed, and 10% cross-reactivity with recombinant human Jagged 2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived rhJagged 1
Ser32-Asp296
Accession # P78504

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Jagged 1 Fc Chimera

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

References

Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
Lai, E.C. (2004) Development 131:965.
Milner, L.A. et al. (1994) Blood 83:2057.
Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
Linheng, L. et al. (1998) Immunity 8:43.
Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
Oda, T. et al. (1997) Nat. Genet. 16:235.
Oda, T. et al. (1997) Genomics 43:376.

Entrez Gene IDs

182 (Human); 16449 (Mouse); 29146 (Rat)

Alternate Names

AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

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Related Research Areas

Citation for Human Jagged 1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1