Human Jagged 1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: Jagged 1
Mammalian Jagged proteins are homologs of the Drosophila Notch ligand, Serrate, and together with Delta-like proteins, constitute ligands for the mammalian Notch proteins. Jagged 1 and 2 are 180 kDa and 150 kDa type I transmembrane glycoproteins, respectively, that possess extracellular DSL (delta, serrate, lag-2 consensus sequence) domains necessary for binding to Notch receptors, along with 15-16 calcium-binding EGF-like repeats which modulate the binding affinity. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis and development of the vasculature. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for functional Notch signaling.
Citation for Human Jagged 1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
Authors: H Kim, B Jang, YJ Kim, JC Choi
International Journal of Molecular Sciences, 2022-09-11;23(18):.
Species: Human
Sample Types: Serum
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We used this kit to quantify the Jagged-1 in human serum and plasma samples. Could not detect any Jagged-1 in our samples but standard curve worked well.
