Human Kallikrein 11 Antibody

Name: Human Kallikrein 11 Antibody
Brand: R&D Systems
SKU: MAB1595

Catalog #: MAB1595 Datasheet / COA / SDS

Discontinued Product

MAB1595 has been discontinued.

View all Kallikrein 11 products.

Kallikrein 11 in Human Breast Cancer Tissue.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 11 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Kallikrein 3 and recombinant human Kallikrein 5 is observed.

Source

Monoclonal Mouse IgG₃ Clone # 189308

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 11
Glu19-Asn250
Accession # Q9UBX7 Isoform 1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose and Mannitol. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Kallikrein 11 (Catalog # 1595-SE)

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Kallikrein 11 in Human Breast Cancer Tissue. Kallikrein 11 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 8 µg/mL Mouse Anti-Human Kallikrein 11 Monoclonal Antibody (Catalog # MAB1595) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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Kallikrein 11 in Human Breast. Kallikrein 11 was detected in immersion fixed paraffin-embedded sections of human breast array using Mouse Anti-Human Kallikrein 11 Monoclonal Antibody (Catalog # MAB1595) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 11

As a member of human tissue kallikrein family, Kallikrein 11, also known as hippostasin, trypsin‑like serine protease and PRSS20, is encoded by the KLK11 gene (1). Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences,respectively (2‑5). Isoform 1 consists of a signal peptide (residues 1‑18), a short pro peptide (residues 19‑21) and the mature chain (residues 22‑250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue 1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas (6‑8).

References

Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Yoshida, S. et al. (1998) Biochim. Biophys. Acta 1399:225.
Yousef, G.M. et al. (2000) Genomics 63:88.
Mitsui, S. et al. (2000) Biochem. Biophys. Res. Commun. 272:205.
Gan, L. et al. (2000) Gene 257:119.
Diamandis, E.P. et al. (2002) Cancer Res. 62:295.
Nakamura, T. et al. (2003) Urology 61:1042.
Borgono, C.A. et al. (2003) Int. J. Cancer 106:605.

Entrez Gene IDs

11012 (Human)

Alternate Names

EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; Hippostasin; hK11; Kallikrein 11; kallikrein-related peptidase 11; KLK11; MGC33060; PRSS20kallikrein-11; Serine protease 20; TLSPhippostasin; Trypsin-like protease