Home / Kallikrein 12 / Human Kallikrein 12 Antibody

Human Kallikrein 12 Antibody

Catalog #: MAB40341
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB40341
MAB40341-SP
Neutralization of Kallikrein 12 Activity by Human Kallikrein 12 Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 12 in direct ELISAs and Western blots. No cross-reactivity with recombinant human (rh)  Kallikrein 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, rhKallikrein  beta 1, rhHGF Activator, rhFactor VII, rhFactor X, rhFactor XI, rhThrombin or rhuPA-1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 364932
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 12
Ala18-Asn248
Accession # Q9UKR0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Kallikrein 12 (Catalog # 3095-SE) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kallikrein 12 (Catalog # 3095-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Kallikrein 12 (0.1 µg/mL, Catalog # 3095-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (0.1 mM, Catalog # ES011). The Neutralization Dose (ND50) is typically 0.1 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Neutralization of Kallikrein 12 Activity by Human Kallikrein 12 Antibody. View Larger

Neutralization of Kallikrein 12 Activity by Human Kallikrein 12 Antibody. The cleavage of Boc-VPR-AMC (0.1 mM, Catalog # ES011) by Recombinant Human Kallikrein 12 (0.1 µg/mL, Catalog # 3095-SE) is measured after preincubation with increasing concentrations of Human Kallikrein 12 Monoclonal Antibody (Catalog # MAB40341). The ND50 is typically 0.1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 12

Human tissue Kallikrein 12, encoded by the KLK12 gene, is a secreted serine protease that belongs to the human tissue kallikrein family (1). It is present in many tissues, such as salivary gland, stomach and breast. KLK12 displays trypsin-like enzymatic activity. This activity can be inhibited by Serpin F2 (R&D Systems, Catalog # 1470-PI). The physiological functions of KLK12 still remain unclear. Its expression is modulated by steroid hormones and is down‑regulated in breast cancer (2). Human KLK12 has three splice variants resulting from alternative splicing of the 3’ end. The amino acid sequence of human KLK12 is 80%, 77%, 71% and 66% to that of bovine, canine, mouse, and rat.

References
  1. Yousef, G.M. et al. (2000) Genomics. 69:331.
  2. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Entrez Gene IDs
43849 (Human)
Alternate Names
DKFZp686H1078; EC 3.4.21; EC 3.4.21.-; Kallikrein 12; Kallikrein-like protein 5; kallikrein-related peptidase 12; KLK12; KLK-L5; KLKL5kallikrein-12; KLK-L5MGC42603

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Citations for Human Kallikrein 12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Kallikrein-Related Peptidase 12 (KLK12) in Breast Cancer as a Favorable Prognostic Marker
    Authors: Sato, A;Takagi, K;Yoshimura, A;Tsukamoto, W;Yamaguchi-Tanaka, M;Miki, Y;Ebata, A;Miyashita, M;Suzuki, T;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin.
    Authors: Bin L, Kim BE, Hall CF
    J. Invest. Dermatol., 2011-07-14;131(11):2213-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Enzymatic properties of human kallikrein-related peptidase 12 (KLK12).
    Authors: Memari</LastName><ForeNam N</Initial, Memari N, Jiang W, Diamandis EP, Luo LY
    Biol. Chem., 2007-04-01;388(4):427-35.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization

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