Human Kallikrein 12 Antibody Summary
Ala18-Asn248
Accession # Q9UKR0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Kallikrein 12 in Human Duodenum. Kallikrein 12 was detected in immersion fixed paraffin-embedded sections of Human Duodenum using Sheep Anti-Human Kallikrein 12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3095) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells in intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 12
Human tissue Kallikrein 12, encoded by the KLK12 gene, is a secreted serine protease that belongs to the human tissue kallikrein family. It is present in many tissues, such as salivary gland, stomach and breast. KLK12 displays trypsin-like enzymatic activity. This activity can be inhibited by Serpin F2 (R&D Systems, Catalog # 1470-PI). The physiological functions of KLK12 still remain unclear. Its expression is modulated by steroid hormones and is down‑regulated in breast cancer (2). Human KLK12 has three splice variants resulting from alternative splicing of the 3’ end. The KLK12 produced by the R&D Systems corresponds to the full-length classical form, also known as isoform 2 (1). The amino acid sequence of human KLK12 is 80%, 77%, 71% and 66% to that of bovine, canine, mouse, and rat. The recombinant human KLK12 can be autoactivated under the conditions described in the Activity Assay Protocol. The active enzyme has the N-terminal sequence of I22FNGTECGRNS.
- Yousef, G.M. et al. (2000) Genomics. 69:331.
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Product Datasheets
Citations for Human Kallikrein 12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Knockdown of KLK12 inhibits viability and induces�apoptosis in human colorectal cancer HT-29 cell line
Authors: Qianyuan Li, Xiukou Zhou, Zhengyu Fang, Huamiao Zhou
International Journal of Molecular Medicine
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Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix
Authors: T Kryza, C Parent, J Pardessus, A Petit, J Burlaud-Ga, P Reverdiau, S Iochmann, V Labas, Y Courty, N Heuzé-Vour
Sci Rep, 2018-04-20;8(1):6331.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Clinical significance of human kallikrein 12 gene expression in gastric cancer
Authors: En-Hao Zhao, Zhi-Yong Shen, Hua Liu, Xin Jin, Hui Cao
World Journal of Gastroenterology
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