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Human Kallikrein 8/Neuropsin Antibody

Catalog #: AF2025
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2025
AF2025-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 8/Neuropsin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 8/Neuropsin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 8
Gln29-Gly260
Accession # O60259
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Kallikrein 8/Neuropsin (Catalog # 2025-SE)
Neutralization
Measured by its ability to neutralize Recombinant Human Kallikrein 8/Neuropsin (0.5 µg/mL, Catalog # 2025-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-Amc (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 8 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 8/Neuropsin

Kallikrein 8 (KLK8), also known as neuropsin or ovasin, is a member of the human tissue kallikrein family (1). Two alternatively spliced forms exist, resulting in 260 (isoform 1) and 305 (isoform 2) amino acid sequences, respectively (2). Isoform 1 consists of a signal peptide (residues 1 to 28), a short pro peptide (residues 29 to 32) and the mature chain (residues 33 to 260). Isoform 2 is identical to isoform 1, except that a 45 amino acid segment is inserted in isoform 2 between residues 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8). This activity can be inhibited by rhSerpin A5, rhSerpin F2 and AEBSF (R&D Systems, Catalog # 1266-PI, 1470-PI and EI001, respectively). Recombinant human KLK8 produced by R&D Systems corresponds to isoform 1.

References
  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Mitsui, S. et al. (1999) Eur. J. Biochem. 260:627.
  3. Hirata, A. et al. (2001) Mol. Cell. Neurosci. 17:600.
  4. Davies, B. et al. (2001) J. Neurosci. 21:6993.
  5. Kishi, T. et al. (2003) Cancer Res. 63:2771.
  6. Cane, S. et al. (2004) Am. J. Obstet. Gynecol. 190:60.
  7. Oka, T. et al. (2002) J. Biol. Chem. 277:14724.
  8. Shimizu, C. et al. (1998) J. Biol. Chem. 273:11189.
Entrez Gene IDs
11202 (Human)
Alternate Names
EC 3.4.21; EC 3.4.21.118; hK8; HNP; kallikrein 8 (neuropsin/ovasin); Kallikrein 8; kallikrein-related peptidase 8; KLK8 protein type 1; KLK8 protein type 2; KLK8; neuropsin type 1; neuropsin type 2; Neuropsin; NP; NRPN; Ovasin; PRSS19; PRSS19kallikrein-8; Serine protease 19; Serine protease TADG-14; TADG14neuropsin; Tumor-associated differentially expressed gene 14 protein

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Citations for Human Kallikrein 8/Neuropsin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Brf1 posttranscriptionally regulates pluripotency and differentiation responses downstream of Erk MAP kinase.
    Authors: Tan, Frederic, Elowitz, Michael
    Proc Natl Acad Sci U S A, 2014-04-14;111(17):E1740-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma.
    Authors: Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD
    Biol. Chem., 2007-11-01;388(11):1235-42.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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