Human KCC2/SLC12A5 Antibody

Catalog #: MAB8369 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8369
MAB8369-SP

Detection of Human KCC2/SLC12A5 by Western Blot.

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All KCC2/SLC12A5 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KCC2/SLC12A5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SLC12A5 in ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 905705

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human SLC12A5
Accession # Q9H2X9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human KCC2/SLC12A5 by Western Blot. Western blot shows lysates of human brain stem tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human KCC2/SLC12A5 Monoclonal Antibody (Catalog # MAB8369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for KCC2/SLC12A5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of KCC2/SLC12A5 in HEK293 Human Cell Line Transfected with Human KCC2/SLC12A5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human KCC2/SLC12A5 and eGFP was stained with either (A) Mouse Anti-Human KCC2/SLC12A5 Monoclonal Antibody (Catalog # MAB8369) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KCC2/SLC12A5

Solute Carrier, family 12 (potassium/chloride transporter), member 5 (SLC12A5) encodes KCC2, a multi-pass co-transporter for K⁺and Cl^-. KCC2/SLC12A5 is expressed on neurons, and is dysregulated in schizophrenia, spasticity, and affective disorders.

Long Name

K-Cl Cotransporter 2

Entrez Gene IDs

57468 (Human); 57138 (Mouse); 171373 (Rat)

Alternate Names

Electroneutral potassium-chloride cotransporter 2; hKCC2; KCC2; KCC2K-Cl cotransporter 2; KIAA1176erythroid K-Cl cotransporter 2; Neuronal K-Cl cotransporter; SLC12A5; solute carrier family 12 (potassium/chloride transporter), member 5; solute carrier family 12 member 5