Human L-Selectin/CD62L DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human L-Selectin/CD62L. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
Normal Goat Serum: (Catalog # DY005)
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Normal Goat Serum: (Catalog # DY005)
Scientific Data
Product Datasheets
Preparation and Storage
Background: L-Selectin/CD62L
The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent with NGS, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Human L-Selectin/CD62L DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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HIV-1 release requires Nef-induced caspase activation
Authors: J Segura, J Ireland, Z Zou, G Roth, J Buchwald, TJ Shen, E Fischer, S Moir, TW Chun, PD Sun
PLoS ONE, 2023-02-13;18(2):e0281087.
Species: Human
Sample Types: Cell Culture Supernates
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L-Selectin/CD62L is a Key Driver of Non-Alcoholic Steatohepatitis in Mice and Men
Authors: HK Drescher, A Schippers, S Rosenhain, F Gremse, L Bongiovann, A Bruin, S Eswaran, SU Gallage, D Pfister, M Szydlowska, M Heikenwald, S Weiskirche, N Wagner, C Trautwein, R Weiskirche, DC Kroy
Cells, 2020-04-29;9(5):.
Species: Human
Sample Types: Serum
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Pleiotropy of ABO gene: correlation of rs644234 with E-selectin and lipid levels
Authors: AA Arguinano, NC Ndiaye, C Masson, S Visvikis-S
Clin. Chem. Lab. Med., 2018-04-25;0(0):.
Species: Human
Sample Types: Plasma
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Over-expression of a human CD62L ecto-domain and a potential role of RNA pseudoknot structures in recombinant protein expression
Authors: M Spencer, N Max, J Ireland, Z Zou, R Wang, P Sun
Protein Expr. Purif., 2017-08-24;140(0):65-73.
Species: Chinese Hamster
Sample Types: Cell Culture Supernates
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TREM-1 SNP rs2234246 regulates TREM-1 protein and mRNA levels and is associated with plasma levels of L-selectin
Authors: AA Aldasoro A, S Dadé, M Stathopoul, M Derive, N Coumba Ndi, T Xie, C Masson, S Gibot, S Visvikis-S
PLoS ONE, 2017-08-03;12(8):e0182226.
Species: Human
Sample Types: Serum
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White matter damage and systemic inflammation in Parkinson's disease
Authors: PL Chiang, HL Chen, CH Lu, PC Chen, MH Chen, IH Yang, NW Tsai, WC Lin
BMC Neurosci, 2017-06-08;18(1):48.
Species: Human
Sample Types: Serum
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Facile supermolecular aptamer inhibitors of L-selectin.
Authors: Chang, Elizabet, Eckert, Mark A, Ali, M Monsur, Riazifar, Hamidrez, Pone, Egest J, Liu, Linan, Zhao, Weian
PLoS ONE, 2015-03-31;10(3):e0123034.
Species: Human
Sample Types: Cell Culture Supernates
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