Human Lactate Dehydrogenase B/LDHB Antibody
Human Lactate Dehydrogenase B/LDHB Antibody Summary
Met1-Val93
Accession # P07195
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Lactate Dehydrogenase B/LDHB by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Lactate Dehydrogenase B/LDHB by Simple WesternTM. Simple Western shows lysates of Exosome Standard (LNCaP) (NBP3-11687) and PC‑3 human prostate cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 38 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
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Lactate Dehydrogenase B/LDHB in Human Liver. Lactate Dehydrogenase B/LDHB was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Lactate Dehydrogenase B/LDHB in HeLa Human Cell Line. Lactate Dehydrogenase B/LDHB was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Lactate Dehydrogenase B/LDHB in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Western Blot Shows Human Lactate Dehydrogenase B/LDHB Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and LDHB knockout HEK293T cell line (KO). PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lactate Dehydrogenase B/LDHB
LDHB is one of two subunits of Lactose Dehydrogenase which is formed from tetramer arrangements of the A and B subunits. LDHB is part of the pyruvate fermentation to lactate pathway in which it catalyzes the conversion of L-lactate and NAD to pyruvate and NADH. LDHA is widely expressed, with high expression in muscle and brain. High expression in tumor cells correlates with low survival rates in various cancers including osteosarcoma, oral squamous cell carcinoma breast and lung cancers. Unlike Lactose Dehyrogenase A, LDHB mutations are not linked to any disease
Product Datasheets
Citations for Human Lactate Dehydrogenase B/LDHB Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Forward genetic screens identify mechanisms of resistance to small molecule lactate dehydrogenase inhibitors
Authors: Frank, AR;Vandiver, F;McFadden, DG;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Role of Lactate in the Regulation of Transcriptional Activity of Breast Cancer-Related Genes and Epithelial-to-Mesenchymal Transition Proteins: A Comparison of MCF7 and MDA-MB-231 Cancer Cell Lines
Authors: San-Millan, I;Martinez, JL;Pickard, SL;Yu, H;Hirsch, FR;Rivard, CJ;Brooks, GA;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
AMPK&alpha loss promotes KRAS-mediated lung tumorigenesis
Authors: M La Montagn, L Shi, P Magee, S Sahoo, M Fassan, M Garofalo
Cell Death and Differentiation, 2021-05-26;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Transcriptional and Metabolic Dissection of ATRA-Induced Granulocytic Differentiation in NB4 Acute Promyelocytic Leukemia Cells
Authors: J Albanesi, NI Noguera, C Banella, T Colangelo, E De Marinis, S Leone, O Palumbo, MT Voso, P Ascenzi, C Nervi, F Bianchi, A di Masi
Cells, 2020-11-05;9(11):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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