Human LAIR1 APC-conjugated Antibody

Catalog #: FAB2664A Datasheet / COA / SDS

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FAB2664A

Detection of LAIR1 in Human Blood Lymphocytes by Flow Cytometry.

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Human LAIR1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LAIR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LAIR2 or recombinant mouse LAIR1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 342219

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAIR1 isoform 1
Gln22-His163
Accession # Q6GTX8

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LAIR1 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human LAIR1 APC-conjugated Monoclonal Antibody (Catalog # FAB2664A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1; also CD305) is a 46 kDa inhibitory receptor belonging to the Ig superfamily. It is a type I transmembrane protein with one extracellular Ig-like domain and two cytoplasmic ITIMs. Four LAIR-1 splice variants exist. LAIR1b has a 17 aa deletion outside the Ig loop in the extracellular domain. It differs from LAIR1c by one aa residue. LAIR1d has a 77 aa truncation in the cytoplasmic domain. LAIR1 is expressed on NK cells, T cells, B cells, monocytes, dendritic cells and most thymocytes. The extracellular domain of human LAIR1 shares 40% aa identity with that of the mouse protein.

Long Name

Leukocyte-associated Ig-like Receptor 1

Entrez Gene IDs

3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)

Alternate Names

CD305 antigen; CD305; CD305leukocyte-associated Ig-like receptor 1; HLAIR1; LAIR1; LAIR-1; leukocyte-associated immunoglobulin-like receptor 1