Human Lamin B1 Antibody Summary
Arg418-Ser582
Accession # P20700
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human Lamin B1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Lamin B1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Lamin B1 in Human Liver. Lamin B1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
View Larger
Lamin B1 in HeLa Human Cell Line. Lamin B1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclear membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lamin B1
Lamins are components of the nuclear
lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear
membrane, which is thought to provide a framework for the nuclear
envelope and may also interact with chromatin. Lamin B1 is a heavily phosphorylated protein, with a calculated molecular weight of 66kDa.
Product Datasheets
Citations for Human Lamin B1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
G Protein-Coupled Receptor 37L1 Modulates Epigenetic Changes in Human Renal Proximal Tubule Cells
Authors: I Armando, S Cuevas, C Fan, M Kumar, Z Izzi, PA Jose, PR Konkalmatt
International Journal of Molecular Sciences, 2022-11-21;23(22):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Identification of a novel tumor angiogenesis inhibitor targeting Shh/Gli1 signaling pathway in Non-small cell lung cancer
Authors: X Lei, Y Zhong, L Huang, S Li, J Fu, L Zhang, Y Zhang, Q Deng, X Yu
Cell Death Dis, 2020-04-14;11(4):232.
Species: Mouse
Sample Types: Protein
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Lamin B1 Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human Lamin B1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
