Human Latrophilin 3/LPHN3 Antibody

Catalog #: AF5916 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5916
AF5916-SP

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Detection of Human Latrophilin 3/LPHN3 by Western Blot.

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Human Latrophilin 3/LPHN3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Latrophilin 3/LPHN3 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Latrophilin 3/LPHN3
Phe20-Asn813
Accession # NP_056051

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Latrophilin 3/LPHN3 by Western Blot. Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Latrophilin 3/ LPHN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5916) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Latrophilin 3/ LPHN3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Latrophilin 3/LPHN3

LPHN3 (Latrophilin 3; also CIRL3, CL3 and LEC3) is a predicted 162 kDa member of the LN-TM7 subfamily, GPCR 2 family of molecules. It appears to have a restricted expression pattern, being limited to the brain and adrenal gland. Although it is related to the black widow toxin receptor CIRL1, it does not serve as a toxin receptor. Mature human LPHN3 is a 7‑TM glycoprotein that is 1428 amino acids (aa) in length. Posttranslational processing cleaves the molecule into a 120 kDa ECD (aa 20‑841) and a noncovalently-associated 85 kDa 7-TM C-terminus (aa 842‑1447). The ECD is modular, and contains a SUEL-like lectin domain (aa 35‑124), an Olf region (aa 134‑393) and GPS domain (aa 802‑853). There are multiple splice events which, in the ECD, include a two aa substitution for aa 127‑131, a 13 aa insertion after Lys623, and a 39 aa substitution for aa 668‑1447. Over aa 20‑813, human LPHN3 shares 98% aa identity with mouse LPHN3.

Entrez Gene IDs

23284 (Human); 319387 (Mouse); 170641 (Rat)

Alternate Names

CIRL3; Latrophilin 3; latrophilin homolog 3 (cow); LEC3; Lectomedin-3; LPHN3