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Human LBP DuoSet ELISA

Catalog #: DY870-05
44 Citations 7 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY870-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human LBP ELISA Standard Curve
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Product Details
Citations (44)
FAQs
Supplemental Products
Reviews (7)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human LBP DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
0.8 - 50 ng/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human LBP. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Human LBP ELISA Standard Curve View Larger

Human LBP ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LBP

Lipopolysaccharide-binding protein (LBP), a 58kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins that includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipidA portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyzes the binding of LPS monomers to CD14, and promotes LPS-induced immune response.

Long Name:
Lipopolysaccharide-binding Protein
Entrez Gene IDs:
3929 (Human); 16803 (Mouse)
Alternate Names:
LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; LPS-binding protein; MGC22233

Citations for Human LBP DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Biomarkers In Prediction of Acute Mesenteric Ischaemia: a prospective multicentre study (BIPAMI study): a study protocol
    Authors: Tamme, K;Acosta, S;Biloslavo, A;Björck, M;Casian, D;Damaskos, D;Forbes, A;Kase, K;Kisand, K;Lakbar, I;Mihnovitš, V;Murruste, M;Mändul, M;Nuzzo, A;Padar, M;Starkopf, J;Visconti, D;Reintam Blaser, A;
    BMC surgery
    Species: Human
    Sample Types: Plasma
  2. Obesity-associated microbiomes instigate visceral adipose tissue inflammation by recruitment of distinct neutrophils
    Authors: Shantaram, D;Hoyd, R;Blaszczak, AM;Antwi, L;Jalilvand, A;Wright, VP;Liu, J;Smith, AJ;Bradley, D;Lafuse, W;Liu, Y;Williams, NF;Snyder, O;Wheeler, C;Needleman, B;Brethauer, S;Noria, S;Renton, D;Perry, KA;Nagareddy, P;Wozniak, D;Mahajan, S;Rana, PSJB;Pietrzak, M;Schlesinger, LS;Spakowicz, DJ;Hsueh, WA;
    Nature communications
    Species: Human
    Sample Types: Plasma
  3. Predictors of HIV rebound differ by timing of antiretroviral therapy initiation
    Authors: Li, JZ;Melberg, M;Kittilson, A;Abdel-Mohsen, M;Li, Y;Aga, E;Bosch, RJ;Wonderlich, ER;Kinslow, J;Giron, LB;Di Germanio, C;Pilkinton, M;MacLaren, L;Keefer, M;Fox, L;Barr, L;Acosta, E;Ananworanich, J;Coombs, R;Mellors, J;Deeks, S;Gandhi, RT;Busch, M;Landay, A;Macatangay, B;Smith, DM;AIDS Clinical Trials Group A5345 Study Team, ;
    JCI insight
    Species: Human
    Sample Types: Plasma
  4. A Blood Immunological Signature of Subclinical Coronary Artery Atherosclerosis in People Living with HIV-1 Receiving Antiretroviral Therapy
    Authors: Wiche Salinas, TR;Zhang, Y;Gosselin, A;Do Rosario, NF;El-Far, M;Filali-Mouhim, A;Routy, JP;Chartrand-Lefebvre, C;Landay, AL;Durand, M;Tremblay, CL;Ancuta, P;Canadian HIV and Aging Cohort Study (CHACS), ;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Plasma
  5. Disrupted balance between pro-inflammatory lipid mediators and anti-inflammatory specialized pro-resolving mediators is linked to hyperinflammation in patients with alcoholic hepatitis
    Authors: Li, W;Xia, Y;Yang, J;Sanyal, AJ;Shah, VH;Chalasani, NP;Yu, Q;
    medRxiv : the preprint server for health sciences
    Species: Human
    Sample Types: Plasma
  6. Genetic variation in environmental enteropathy and stunting in Zambian children: A pilot genome wide association study using the H3Africa chip
    Authors: Mweetwa, MN;Haritunians, T;Dube, S;Chandwe, K;Amadi, B;Zyambo, K;Liu, TC;McGovern, D;Kelly, P;
    PloS one
    Species: Human
    Sample Types: Plasma
  7. Milk-Fat-Globule-Membrane-Enriched Dairy Milk Compared with a Soy-Lecithin-Enriched Beverage Did Not Adversely Affect Endotoxemia or Biomarkers of Gut Barrier Function and Cardiometabolic Risk in Adults with Metabolic Syndrome: A Randomized Controlled Crossover Trial
    Authors: Pokala, A;Quarles, WR;Ortega-Anaya, J;Jimenez-Flores, R;Cao, S;Zeng, M;Hodges, JK;Bruno, RS;
    Nutrients
    Species: Human
    Sample Types: Serum
  8. Serum TGF-?1 and CD14 Predicts Response to Anti-TNF-? Therapy in IBD
    Authors: Coufal, S;Kverka, M;Kreisinger, J;Thon, T;Rob, F;Kolar, M;Reiss, Z;Schierova, D;Kostovcikova, K;Roubalova, R;Bajer, L;Jackova, Z;Mihula, M;Drastich, P;Tresnak Hercogova, J;Novakova, M;Vasatko, M;Lukas, M;Tlaskalova-Hogenova, H;Jiraskova Zakostelska, Z;
    Journal of immunology research
    Species: Human
    Sample Types: Serum
  9. Increased plasma lipopolysaccharide-binding protein and altered inflammatory mediators in overweight women suggest a state of subclinical endotoxemia
    Authors: Metz, CN;Xue, X;Chatterjee, PK;Adelson, R;Brines, M;Tracey, KJ;Gregersen, PK;Pavlov, VA;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Plasma
  10. Intestinal Barrier Dysfunction and Microbial Translocation in Patients with First-Diagnosed Atrial Fibrillation
    Authors: L Blöbaum, M Witkowski, M Wegner, S Lammel, PA Schencke, K Jakobs, M Puccini, D Rei beta ner, D Steffens, U Landmesser, U Rauch, J Friebel
    Biomedicines, 2023-01-10;11(1):.
    Species: Human
    Sample Types: Plasma
  11. The biological activity of serum bacterial lipopolysaccharides associates with disease activity and likelihood of achieving remission in patients with rheumatoid arthritis
    Authors: J Parantaine, G Barreto, R Koivuniemi, H Kautiainen, D Nordström, E Moilanen, M Hämäläinen, M Leirisalo-, K Nurmi, KK Eklund
    Arthritis Research & Therapy, 2022-11-21;24(1):256.
    Species: Human
    Sample Types: Serum
  12. Intestinal Injury Biomarkers Predict Mortality in Pediatric Severe Malaria
    Authors: ML Sarangam, R Namazzi, D Datta, C Bond, CPB Vanderpool, RO Opoka, CC John, AL Conroy
    MBio, 2022-09-07;0(0):e0132522.
    Species: Human
    Sample Types: Serum
  13. Immune responses in Omicron SARS-CoV-2 breakthrough infection in vaccinated adults
    Authors: H Kared, AS Wolf, A Alirezayla, A Ravussin, G Solum, TT Tran, F Lund-Johan, JT Vaage, LS Nissen-Mey, UC Nygaard, O Hungnes, AH Robertson, LM Næss, L Trogstad, P Magnus, LA Munthe, S Mjaaland
    Nature Communications, 2022-07-18;13(1):4165.
    Species: Human
    Sample Types: Plasma
  14. Chitin-glucan supplementation improved postprandial metabolism and altered gut microbiota in subjects at cardiometabolic risk in a randomized trial
    Authors: H Ranaivo, Z Zhang, M Alligier, L Van Den Be, M Sothier, S Lambert-Po, N Feugier, C Cuerq, C Machon, AM Neyrinck, B Seethaler, J Rodriguez, M Roumain, GG Muccioli, V Maquet, M Laville, SC Bischoff, J Walter, NM Delzenne, JA Nazare
    Scientific Reports, 2022-05-25;12(1):8830.
    Species: Human
    Sample Types: Feces
  15. "HIIT the Inflammation": Comparative Effects of Low-Volume Interval Training and Resistance Exercises on Inflammatory Indices in Obese Metabolic Syndrome Patients Undergoing Caloric Restriction
    Authors: D Reljic, W Dieterich, HJ Herrmann, MF Neurath, Y Zopf
    Nutrients, 2022-05-10;14(10):.
    Species: Human
    Sample Types: Serum
  16. Liver alterations are not improved by inulin supplementation in alcohol use disorder patients during alcohol withdrawal: A pilot randomized, double-blind, placebo-controlled study
    Authors: C Amadieu, L Maccioni, S Leclercq, AM Neyrinck, NM Delzenne, P de Timary, P Stärkel
    EBioMedicine, 2022-04-28;80(0):104033.
    Species: Human
    Sample Types: Plasma
  17. Gut Microbial Signatures of Distinct Trimethylamine N-Oxide Response to Raspberry Consumption
    Authors: M Franck, J de Toro-Ma, T V Varin, V Garneau, G Pilon, D Roy, P Couture, C Couillard, A Marette, MC Vohl
    Nutrients, 2022-04-15;14(8):.
    Species: Human
    Sample Types: Plasma
  18. Plasma and fecal zonulin are not altered by a high green leafy vegetable dietary intervention: secondary analysis of a randomized control crossover trial
    Authors: AJ Riviere, KS Smith, MN Schaberg, MW Greene, AD Frugé
    BMC gastroenterology, 2022-04-12;22(1):184.
    Species: Human
    Sample Types: Serum
  19. Intestinal permeability before and after albendazole treatment in low and high socioeconomic status schoolchildren in Makassar, Indonesia
    Authors: AI Amaruddin, JPR Koopman, M Muhammad, K Lenaerts, HMH van Eijk, EAT Brienen, AR Geelen, L van Liesho, S Wahyuni, EJ Kuijper, RD Zwittink, F Hamid, E Sartono, M Yazdanbakh
    Scientific Reports, 2022-03-01;12(1):3394.
    Species: Human
    Sample Types: Serum
  20. Gut- and oral-dysbiosis differentially impact spinal- and bulbar-onset ALS, predicting ALS severity and potentially determining the location of disease onset
    Authors: HS Kim, J Son, D Lee, J Tsai, D Wang, ES Chocron, S Jeong, P Kittrell, CF Murchison, RE Kennedy, A Tobon, CE Jackson, AM Pickering
    BMC neurology, 2022-02-21;22(1):62.
    Species: Human
    Sample Types: Plasma
  21. Antibody microarray analysis of amniotic fluid proteomes in women with cervical insufficiency and short cervix, and their association with pregnancy latency length
    Authors: S Hong, KH Park, YE Lee, JE Lee, YM Kim, E Joo, I Cho
    PLoS ONE, 2022-02-07;17(2):e0263586.
    Species: Human
    Sample Types: Amniotic Fluid
  22. Shift Work Predicts Increases in Lipopolysaccharide-Binding Protein, Interleukin-10, and Leukocyte Counts in a Cross-Sectional Study of Healthy Volunteers Carrying Low-Grade Systemic Inflammation
    Authors: AQ Atwater, LC Immergluck, AJ Davidson, O Castanon-C
    International Journal of Environmental Research and Public Health, 2021-12-14;18(24):.
    Species: Human
    Sample Types: Plasma
  23. Dysbiosis of Gut Microbiota Promotes Hepatocellular Carcinoma Progression by Regulating the Immune Response
    Authors: N Zhang, Y Gou, S Liang, N Chen, Y Liu, Q He, J Zhang
    Journal of Immunology Research, 2021-10-20;2021(0):4973589.
    Species: Human
    Sample Types: Plasma
  24. Circulating bioactive bacterial DNA is associated with immune activation and complications in common variable immunodeficiency
    Authors: HE Ho, L Radigan, G Bongers, A El-Shamy, C Cunningham
    JCI Insight, 2021-10-08;6(19):.
    Species: Human
    Sample Types: Serum
  25. Prebiotic Inulin Supplementation and Peripheral Insulin Sensitivity in adults at Elevated Risk for Type 2 Diabetes: A Pilot Randomized Controlled Trial
    Authors: CM Mitchell, BM Davy, MA Ponder, RP McMillan, MD Hughes, MW Hulver, AP Neilson, KP Davy
    Nutrients, 2021-09-17;13(9):.
    Species: Human
    Sample Types:
  26. Gut Leakage Markers in Response to Strenuous Exercise in Patients with Suspected Coronary Artery Disease
    Authors: SK Aune, J Cwikiel, A Flaa, H Arnesen, S Solheim, A Awoyemi, M Trøseid, I Seljeflot, R Helseth
    Cells, 2021-08-25;10(9):.
    Species: Human
    Sample Types: Plasma
  27. Adaptation of the small intestine to microbial enteropathogens in Zambian children with stunting
    Authors: B Amadi, K Zyambo, K Chandwe, E Besa, C Mulenga, S Mwakamui, S Siyumbwa, S Croft, R Banda, M Chipunza, K Chifunda, L Kazhila, K VanBuskirk, P Kelly
    Nature Microbiology, 2021-02-15;0(0):.
    Species: Human
    Sample Types: Plasma
  28. Association of serum lipopolysaccharide-binding protein level with sensitization to food allergens in children
    Authors: EK Ha, JH Kim, DK Yon, SW Lee, MA Kim, KS Lee, M Sung, HM Jee, YH Shin, MY Han
    Scientific Reports, 2021-01-25;11(1):2143.
    Species: Human
    Sample Types: Serum
  29. Elevated acute phase proteins reflect peripheral inflammation and disease severity in patients with amyotrophic lateral sclerosis
    Authors: DR Beers, W Zhao, DW Neal, JR Thonhoff, AD Thome, A Faridar, S Wen, J Wang, SH Appel
    Scientific Reports, 2020-09-17;10(1):15295.
    Species: Human
    Sample Types: Serum
  30. High microbial translocation limits gut immune recovery during short-term HAART in the area with high prevalence of foodborne infection
    Authors: D Kantamala, J Praparatta, S Taejaroenk, S Srithep, R Yoosupap, K Supparatpi
    Cytokine, 2020-08-26;136(0):155257.
    Species: Human
    Sample Types: Plasma
  31. Effects of Probiotic Strains on Disease Activity and Enteric Permeability in Psoriatic Arthritis-A Pilot Open-Label Study
    Authors: A Haidmayer, P Bosch, A Lackner, M D'Orazio, J Fessler, MH Stradner
    Nutrients, 2020-08-05;12(8):.
    Species: Human
    Sample Types: Plasma
  32. Permissive microbiome characterizes human subjects with a neurovascular disease cavernous angioma
    Authors: SP Polster, A Sharma, C Tanes, AT Tang, P Mericko, Y Cao, J Carrión-Pe, R Girard, J Koskimäki, D Zhang, A Stadnik, SG Romanos, SB Lyne, R Shenkar, K Yan, C Lee, A Akers, L Morrison, M Robinson, A Zafar, K Bittinger, H Kim, JA Gilbert, ML Kahn, L Shen, IA Awad
    Nat Commun, 2020-05-27;11(1):2659.
    Species: Human
    Sample Types: Plasma
  33. Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection
    Authors: LB Giron, CE Tanes, MH Schleimann, PA Engen, LM Mattei, A Anzurez, M Damra, H Zhang, K Bittinger, F Bushman, A Kossenkov, PW Denton, H Tateno, A Keshavarzi, AL Landay, M Abdel-Mohs
    Mucosal Immunol, 2020-03-09;0(0):.
    Species: Human
    Sample Types: Plasma
  34. The effects of a multispecies synbiotic on microbiome-related side effects of long-term proton pump inhibitor use: A pilot study
    Authors: A Horvath, B Leber, N Feldbacher, M Steinwende, I Komarova, F Rainer, A Blesl, V Stadlbauer
    Sci Rep, 2020-02-17;10(1):2723.
    Species: Human
    Sample Types: Plasma
  35. Glycosylation deficiency of lipopolysaccharide-binding protein and corticosteroid-binding globulin associated with activity and response to treatment for rheumatoid arthritis
    Authors: F Ciregia, D Baiwir, G Cobraivill, T Dewael, G Mazzucchel, V Badot, S Di Romana, P Sidiras, T Sokolova, P Durez, MG Malaise, D de Seny
    J Transl Med, 2020-01-06;18(1):8.
    Species: Human
    Sample Types: Serum
  36. Effects of glepaglutide, a novel long-acting glucagon-like peptide-2 analogue, on markers of liver status in patients with short bowel syndrome: findings from a randomised phase 2 trial
    Authors: RM Naimi, M Hvistendah, N Nerup, R Ambrus, MP Achiam, LB Svendsen, H Grønbæk, HJ Møller, H Vilstrup, A Steensberg, PB Jeppesen
    EBioMedicine, 2019-07-17;0(0):.
    Species: Human
    Sample Types: Plasma
  37. Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response
    Authors: S Coufal, N Galanova, L Bajer, Z Gajdarova, D Schierova, Z Jiraskova, K Kostovciko, Z Jackova, Z Stehlikova, P Drastich, H Tlaskalova, M Kverka
    Cells, 2019-07-13;8(7):.
    Species: Human
    Sample Types: Serum
  38. Effects of Tobacco Usage and Antiretroviral Therapy on Biomarkers of Systemic Immune Activation in HIV-Infected Participants
    Authors: HC Steel, WDF Venter, AJ Theron, R Anderson, C Feldman, L Kwofie, T Cronjé, N Arullapan, TM Rossouw
    Mediators Inflamm., 2018-12-09;2018(0):8357109.
    Species: Human
    Sample Types: Plasma
  39. The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages
    Authors: C Petes, V Mintsopoul, RL Finnen, BW Banfield, K Gee
    J. Biol. Chem., 2018-09-21;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Markers of metabolic endotoxemia as related to metabolic syndrome in an elderly male population at high cardiovascular risk: a cross-sectional study
    Authors: A Awoyemi, M Trøseid, H Arnesen, S Solheim, I Seljeflot
    Diabetol Metab Syndr, 2018-07-21;10(0):59.
    Species: Human
    Sample Types: Plasma
  41. The level of lipopolysaccharide-binding protein is elevated in adult patients with obstructive sleep apnea
    Authors: Y Kong, Z Li, T Tang, H Wu, J Liu, L Gu, T Zhao, Q Huang
    BMC Pulm Med, 2018-05-29;18(1):90.
    Species: Human
    Sample Types: Serum
  42. Rifaximin Reduces Markers of Inflammation and Bacterial 16S rRNA in Zambian Adults with Hepatosplenic Schistosomiasis: A Randomized Control Trial
    Authors: E Sinkala, K Zyambo, E Besa, P Kaonga, B Nsokolo, V Kayamba, M Vinikoor, R Zulu, M Bwalya, GR Foster, P Kelly
    Am. J. Trop. Med. Hyg., 2018-02-08;0(0):.
    Species: Human
    Sample Types: Plasma
  43. Bacteroides are associated with GALT iNKT cell function and reduction of microbial translocation in HIV-1 infection
    Authors: D Paquin-Pro, C Ching, I Vujkovic-C, D Fadrosh, L Loh, Y Huang, M Somsouk, SV Lynch, PW Hunt, DF Nixon, D SenGupta
    Mucosal Immunol, 2016-04-06;0(0):.
    Species: Human
    Sample Types: Plasma
  44. Low levels of microbial translocation marker LBP are associated with sustained viral response after anti-HCV treatment in HIV-1/HCV co-infected patients.
    Authors: Nystrom J, Stenkvist J, Haggblom A, Weiland O, Nowak P
    PLoS ONE, 2015-03-18;10(3):e0118643.
    Species: Human
    Sample Types: Plasma

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Reviews for Human LBP DuoSet ELISA

Average Rating: 4.7 (Based on 7 Reviews)

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Human LBP DuoSet ELISA
By Justyna Lopatecka on 06/25/2024
Sample Tested: Purified Standard
Human LBP DuoSet ELISA DY870-05
Human LBP DuoSet ELISA
By Anonymous on 03/16/2023
Sample Tested: EDTA Plasma,Urine

There appeared to be a urine matrix effect at 2-fold dilution. Tested plasma at 200-fold dilution and values were above the standard curve range.

Human LBP DuoSet ELISA DY870-05
Human LBP DuoSet ELISA
By Anonymous on 03/24/2021
Sample Tested: EDTA Plasma
Human LBP DuoSet ELISA DY870-05
Human LBP DuoSet ELISA
By Anonymous on 06/09/2020
Sample Tested: Serum and Plasma

We used this kit to quantify the LBP in human serum and plasma samples. Works very well and generates a good standard curve.

Human LBP DuoSet ELISA DY870-05
Human LBP DuoSet ELISA
By Anonymous on 01/23/2020
Sample Tested: EDTA Plasma

Performance and reproducibility of the kit are very good (see graph attached). Also, very good customer service and fast shipment.

On the downside, the protocol is tricky to undstand.The information to perform the test is spread on three documents (the protocol, the certificate of analysis and the list of materials), so that pooling this information gives you quite the extra work.

Human LBP DuoSet ELISA DY870-05
Human LBP DuoSet ELISA
By Anonymous on 10/03/2018
Sample Tested: EDTA Plasma
Human LBP DuoSet ELISA DY870-05
Human LBP DuoSet ELISA
By Anonymous on 10/03/2018
Sample Tested: EDTA Plasma
Human LBP DuoSet ELISA DY870-05