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Human Leptin/OB Antibody

Catalog #: MAB398
21 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB398-SP
MAB398-100
MAB398-500
Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody.
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Citations (21)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Leptin/OB Antibody Summary

Species Reactivity
Human
Specificity
Captures human Leptin/OB in sandwich ELISAs. In this format, this antibody shows less than 0.1% cross-reactivity with recombinant mouse (rm) Leptin/OB and rrLeptin/OB.
Source
Monoclonal Mouse IgG1 Clone # 44802
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Leptin/OB
Val22-Cys167
Accession # P41159
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample

Human Leptin/OB Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Leptin/OB Biotinylated Antibody (Catalog # BAM398)

Standard: Recombinant Human Leptin Protein, CF (Catalog # 398-LP)

Neutralization
Measured by its ability to neutralize Leptin/OB-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R. The Neutralization Dose (ND50) is typically 0.4-1.6 µg/mL in the presence of 7.5 ng/mL Recombinant Human Leptin/OB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody. View Larger

Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody. Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Human Leptin/OB Monoclonal Antibody (Catalog # MAB398). The ND50 is typically 0.4-1.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.

References
  1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
  2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Entrez Gene IDs
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

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Citations for Human Leptin/OB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Dopamine Receptors in Human Adipocytes: Expression and Functions
    Authors: Dana C. Borcherding, Eric R. Hugo, Gila Idelman, Anuradha De Silva, Nathan W. Richtand, Jean Loftus et al.
    PLoS ONE
  2. Induction of insulin resistance by the adipokines resistin, leptin, plasminogen activator inhibitor-1 and retinol binding protein 4 in human megakaryocytes
    Authors: Anja J Gerrits, Eelo Gitz, Cornelis A Koekman, Frank L Visseren, Timon W van Haeften, Jan Willem N Akkerman
    Haematologica
  3. Extra-adrenal glucocorticoids contribute to the postprandial increase of circulating leptin in mice
    Authors: Yuka Tomabechi, Takeshi Tsuruta, Shinichi Saito, Martin Wabitsch, Kei Sonoyama
    Journal of Cell Communication and Signaling
  4. GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans
    Authors: Satish Patel, Anna Alvarez-Guaita, Audrey Melvin, Debra Rimmington, Alessia Dattilo, Emily L. Miedzybrodzka et al.
    Cell Metabolism
  5. Elevated fatty acid beta-oxidation by leptin contributes to the proinflammatory characteristics of fibroblast-like synoviocytes from RA patients via LKB1-AMPK pathway
    Authors: J Wei, X Huang, X Zhang, G Chen, C Zhang, X Zhou, J Qi, Y Zhang, X Li
    Cell Death & Disease, 2023-02-09;14(2):97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Appetite Control across the Lifecourse: The Acute Impact of Breakfast Drink Quantity and Protein Content. The Full4Health Project
    Authors: DR Crabtree, W Buosi, CL Fyfe, GW Horgan, Y Manios, O Androutsos, A Giannopoul, G Finlayson, K Beaulieu, CL Meek, JJ Holst, KV Van Norren, JG Mercer, AM Johnstone
    Nutrients, 2020-11-30;12(12):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA (Capture)
  7. Digital microfluidic assay for protein detection.
    Authors: Mok J, Mindrinos M, Davis R, Javanmard M
    Proc Natl Acad Sci U S A, 2014-01-21;111(6):2110-5.
    Species: N/A
    Sample Types: Beads
    Applications: Immunoassay Development
  8. Regulatory T cell proliferative potential is impaired in human autoimmune disease.
    Authors: Carbone F, De Rosa V, Carrieri P, Montella S, Bruzzese D, Porcellini A, Procaccini C, La Cava A, Matarese G
    Nat Med, 2013-12-08;20(1):69-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012-02-17;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  10. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  11. Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
    Authors: Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B
    Metab. Clin. Exp., 2009-07-01;58(7):946-53.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  12. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  13. Leptin, blood pressure, and aerobic capacity in women.
    Authors: Sabatier MJ, McCully KK, Marinik EL, Schwark EH, Haddow S, Cortez-Cooper M, Bergeron MF, Sloan GJ, Cannon JG
    Am. J. Hypertens., 2008-08-28;21(11):1245-50.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  14. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
    Authors: Emsley HC, Smith CJ, Gavin CM
    BMC Neurol, 2007-02-28;7(0):5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  15. Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    J. Assist. Reprod. Genet., 2005-06-01;22(6):257-64.
    Species: Human
    Sample Types: Follicular Fluid
    Applications: ELISA Development
  16. Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
    Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
    Eur. Cytokine Netw., 2002-04-01;13(2):225-9.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  17. The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements
    Authors: Claudio Procaccini, Fortunata Carbone, Dario Di Silvestre, Francesca Brambilla, Veronica De Rosa, Mario Galgani et al.
    Immunity
  18. Epitope Mapping of Mono- and Polyclonal Antibodies by Screening Phage-displayed Random Peptide Libraries
    Authors: P Molek, T Bratkovi?
    Acta Chim Slov, 2016-12-01;63(4):914-919.
  19. A potential role for hepcidin in obesity-driven colorectal tumourigenesis
    Authors: Elisabeth Phillips, Richard D Horniblow, Vikki Poole, Matthew Bedford, Douglas G Ward, Amanda J Kirkham et al.
    Oncology Reports
  20. Hyperbaric Oxygen Exposure Attenuates Circulating Stress Biomarkers: A Pilot Interventional Study
    Authors: Jae Seung Chang, Eunha Chang, Yoonsuk Lee, Yong Sung Cha, Seung-Kuy Cha, Won Gil Cho et al.
    International Journal of Environmental Research and Public Health
  21. Characterization of an Acellular Scaffold for a Tissue Engineering Approach to the Nipple-Areolar Complex Reconstruction
    Authors: Nicholas C. Pashos, Michelle E. Scarritt, Zachary R. Eagle, Jeffrey M. Gimble, Abigail E. Chaffin, Bruce A. Bunnell
    Cells Tissues Organs (Print)

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Human Leptin/OB Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human