Human LILRB4/CD85k/ILT3 Antibody

Catalog #: MAB24252 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB24252-100
MAB24252-SP

Detection of LILRB4/CD85k/ILT3 in Transfected & Wild Type HEK293.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRB4/CD85k/ILT3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRB4/CD85k/ILT3 in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1057706

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived recombinant human LILRB4/CD85k/ILT3 protein
Pro17-His257
Accession # ABM86208

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

PBMCs with CD123 costain

Immunocytochemistry

3-25 µg/mL

Immersion fixed Transfected & Wild Type HEK293 Human Embryonic Kidney Cell Line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of LILRB4/CD85k/ILT3 in Transfected & Wild Type HEK293. LILRB4/CD85k/ILT3 was detected in immersion fixed Transfected & Wild Type HEK293 Human Embryonic Kidney Cell Line using Mouse Anti-Human LILRB4/CD85k/ILT3 Monoclonal Antibody (Catalog # MAB24252) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry

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Detection of LILRB4/CD85k/ILT3 in PBMCs with CD123 costain cells by Flow Cytometry. PBMCs with CD123 costain were stained with Mouse Anti-Human IL-3R alpha/CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human LILRB4/CD85k/ILT3 Monoclonal Antibody (Catalog # MAB24252) or (B) Mouse IgG_2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB4/CD85k/ILT3

ILT3, also known as CD85k and LIR-5, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation (1). Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). Alternative splicing of human ILT3 generates an isoform that lacks the first ITIM and a secreted isoform that circulates in the serum of cancer patients (3, 4). ILT3 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC) (2, 5, 6). Ligation of ILT3 triggers ITIM-mediated inhibition of cell-activating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection (2, 4, 7, 8). Soluble ILT3 induces the differentiation of CD8⁺ T suppressor cells (Ts) that can inhibit the effector functions of CD4⁺ Th cells and CD8⁺ CTL (4, 7, 9). In turn, CD8⁺ Ts cells induce ILT3 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC (5, 6, 8, 10, 11).

References

Vlad, G. et al. (2010) Int. Rev. Immunol. 29:119.
Cella, M. et al. (1997) J. Exp. Med. 185:1743.
Heinzmann, A. et al. (2000) Eur. J. Immunogenet. 27:121.
Suciu-Foca, N. et al. (2007) J. Immunol. 178:7432.
Gleissner, C.A. et al. (2007) Eur. J. Immunol. 37:177.
Manavalan, J.S. et al. (2004) Int. Immunol. 16:1055.
Vlad, G. and N. Suciu-Foca (2012) Exp. Mol. Pathol. 93:294.
Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
Vlad, G. et al. (2006) Int. Immunopharmacol. 6:1889.
Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
Brenk, M. et al. (2009) J. Immunol. 183:145.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4

Entrez Gene IDs

11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)

Alternate Names

CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4