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Human LILRB4/CD85k/ILT3 APC-conjugated Antibody

Catalog #: FAB24251A
1 Citation 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB24251A-025
FAB24251A-100
Detection of LILRB4/CD85k/ILT3 in Human PBMCs by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRB4/CD85k/ILT3 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ILT3/CD85k in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with with recombinant human (rh) ILT5 is observed. No cross-reactivity with rhILT1, rhILT2, rhILT4, rhILT7, rhILT11, rhLIR-6 or rhLIR-8 is observed.
Source
Monoclonal Mouse IgG2A Clone # 293623
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ILT3/CD85k
Pro17-His257
Accession # ABM86208
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LILRB4/CD85k/ILT3 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of LILRB4/CD85k/ILT3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL-3 Ra/CD123 PE-conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human LILRB4/CD85k/ILT3 APC-conjugated Monoclonal Antibody (Catalog # FAB24251A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRB4/CD85k/ILT3

Immunoglobulin-Like Transcript 3 (ILT3), also known as Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4) and Leukocyte Immunoglobulin-like Receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
Entrez Gene IDs
11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)
Alternate Names
CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4

Product Datasheets

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Citation for Human LILRB4/CD85k/ILT3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Integrating Proteomics and Transcriptomics for Systematic Combinatorial Chimeric Antigen Receptor Therapy of AML.
    Authors: Perna F, Berman S H et al.
    Cancer Cell

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Human LILRB4/CD85k/ILT3 APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100

Flow Cytometry Human LILRB4/CD85k/ILT3 APC‑conjugated Antibody FAB24251A