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Human LILRB4/CD85k/ILT3 PE-conjugated Antibody

Catalog #: FAB24251P
7 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB24251P-025
FAB24251P-100
Detection of LILRB4/CD85k/ILT3 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRB4/CD85k/ILT3 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ILT3/CD85k in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with with recombinant human (rh) ILT5 is observed. No cross-reactivity with rhILT1, rhILT2, rhILT4, rhILT7, rhILT11, rhLIR-6 or rhLIR-8 is observed.
Source
Monoclonal Mouse IgG2A Clone # 293623
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ILT3/CD85k
Pro17-His257
Accession # ABM86208
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LILRB4/CD85k/ILT3 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of LILRB4/CD85k/ILT3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL-3 Ra/CD123 APC-conjugated Monoclonal Antibody (Catalog # FAB301A) and either (A) Mouse Anti-Human LILRB4/CD85k/ILT3 PE-conjugated Monoclonal Antibody (Catalog # FAB24251P) or (B) Mouse IgG2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRB4/CD85k/ILT3

Immunoglobulin-Like Transcript 3 (ILT3), also known as Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4) and Leukocyte Immunoglobulin-like Receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
Entrez Gene IDs
11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)
Alternate Names
CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4

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Citations for Human LILRB4/CD85k/ILT3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Trichinella spiralis Excretory-Secretory Products Induce Tolerogenic Properties in Human Dendritic CellsviaToll-Like Receptors 2 and 4
    Authors: N Ilic, A Gruden-Mov, J Cvetkovic, S Tomic, DB Vucevic, C Aranzamend, M Colic, E Pinelli, L Sofronic-M
    Front Immunol, 2018-01-24;9(0):11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Tolerogenic Dendritic Cells from Poorly Compensated Type 1 Diabetes Patients Have Decreased Ability To Induce Stable Antigen-Specific T Cell Hyporesponsiveness and Generation of Suppressive Regulatory T Cells
    Authors: Klára Dá?ová
    J. Immunol, 2016-12-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Immune suppression and resistance mediated by constitutive activation of Wnt/beta-catenin signaling in human melanoma cells.
    J. Immunol., 2012-07-18;189(5):2110-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae.
    Authors: Zimmer A, Luce S, Gaignier F, Nony E, Naveau M, Biola-Vidamment A, Pallardy M, Van Overtvelt L, Mascarell L, Moingeon P
    J. Immunol., 2011-03-02;186(7):3966-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Phenotypic and functional markers for 1alpha,25-dihydroxyvitamin D(3)-modified regulatory dendritic cells.
    Authors: Pedersen AW, Holmstrom K, Jensen SS, Fuchs D, Rasmussen S, Kvistborg P, Claesson MH, Zocca MB
    Clin. Exp. Immunol., 2009-07-01;157(1):48-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Maturation of dendritic cells depends on proteolytic cleavage by cathepsin X.
    Authors: Obermajer N, Svajger U, Bogyo M, Jeras M, Kos J
    J. Leukoc. Biol., 2008-08-13;84(5):1306-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals.
    Authors: Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS
    J. Exp. Med., 2008-07-14;205(8):1797-805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human LILRB4/CD85k/ILT3 PE-conjugated Antibody

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Human LILRB4/CD85k/ILT3 PE-conjugated Antibody
By Anonymous on 05/10/2023
Application: Flow Sample Tested: HEK293 human embryonic kidney cell line Species: Human
Flow Cytometry Human LILRB4/CD85k/ILT3 PE-conjugated Antibody FAB24251P