Human LRG1 Antibody

Catalog # Availability Size / Price Qty
AF7890
AF7890-SP
Detection of Human LRG1 by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human LRG1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRG1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human LRG1
Val36-Gln347
Accession # P02750
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human LRG1 antibody by Western Blot. View Larger

Detection of Human LRG1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7890) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRG1 at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry LRG1 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

LRG1 in Human Brain. LRG1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7890) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRG1

LRG-1 (Leucine-Rich alpha-2-Glycoprotein 1; also LRHG [in rodent]) is a 45-55 kDa, monomeric, variably glycosylated, secreted protein member of the leucine-rich repeat family of molecules. It should not be confused with either LRIG-1/ Leucine-Rich repeats and Ig-like domains-1, or LRG1/LIM-RhoGap protein 1. LRG-1 has a limited expression pattern, being associated with neutrophils, neutrophil precursors (myelocytes; metamyelocytes), astrocytes, HEV (in mice), hepatocytes and select tumor cells. LRG-1 is reported to bind multiple molecules, including TGF-beta, extracellular cytochrome C, and fibronectin plus collagen IV of the ECM. Functions associated with this binding include granulocyte maturation, protection from apoptosis, and cell migration. Mature human LRG-1 is 312 amino acids (aa) in length (aa 35-347). Depending upon the source, LRG-1 has been described as having either thirteen 24 aa leucine-rich repeats (LRRs), or eight LRRs spanning aa 93-282 coupled to a C-terminal LRR. Variable O- and N-based glycosylation accounts for the variablility in MW. Mature human LRG-1 shares 66% aa sequence identity with mouse LRG-1.

Long Name
Leucine-rich alpha-2-glycoprotein 1
Entrez Gene IDs
116844 (Human); 76905 (Mouse); 367455 (Rat)
Alternate Names
1300008B03Rik; 2310031E04Rik; HMFT1766; leucine-rich alpha-2-glycoprotein 1; leucine-rich alpha-2-glycoprotein; LRG1; LRGleucine rich alpha 2 glycoprotein

Product Datasheets

You must select a language.

x

Citation for Human LRG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Biomarker Panel for the Diagnosis of Pancreatic Ductal Adenocarcinoma
    Authors: H Kim, KN Kang, YS Shin, Y Byun, Y Han, W Kwon, CW Kim, JY Jang
    Cancers (Basel), 2020-06-01;12(6):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA (Capture)

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human LRG1 Antibody

There are currently no reviews for this product. Be the first to review Human LRG1 Antibody and earn rewards!

Have you used Human LRG1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review