Human LRG1 Antibody Summary
Val36-Gln347
Accession # P02750
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human LRG1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7890) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRG1 at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LRG1 in Human Brain. LRG1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7890) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRG1
LRG-1 (Leucine-Rich alpha-2-Glycoprotein 1; also LRHG [in rodent]) is a 45-55 kDa, monomeric, variably glycosylated, secreted protein member of the leucine-rich repeat family of molecules. It should not be confused with either LRIG-1/ Leucine-Rich repeats and Ig-like domains-1, or LRG1/LIM-RhoGap protein 1. LRG-1 has a limited expression pattern, being associated with neutrophils, neutrophil precursors (myelocytes; metamyelocytes), astrocytes, HEV (in mice), hepatocytes and select tumor cells. LRG-1 is reported to bind multiple molecules, including TGF-beta, extracellular cytochrome C, and fibronectin plus collagen IV of the ECM. Functions associated with this binding include granulocyte maturation, protection from apoptosis, and cell migration. Mature human LRG-1 is 312 amino acids (aa) in length (aa 35-347). Depending upon the source, LRG-1 has been described as having either thirteen 24 aa leucine-rich repeats (LRRs), or eight LRRs spanning aa 93-282 coupled to a C-terminal LRR. Variable O- and N-based glycosylation accounts for the variablility in MW. Mature human LRG-1 shares 66% aa sequence identity with mouse LRG-1.
Product Datasheets
Citation for Human LRG1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Biomarker Panel for the Diagnosis of Pancreatic Ductal Adenocarcinoma
Authors: H Kim, KN Kang, YS Shin, Y Byun, Y Han, W Kwon, CW Kim, JY Jang
Cancers (Basel), 2020-06-01;12(6):.
Species: Human
Sample Types: Plasma
Applications: ELISA (Capture)
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